Restriction fragment length polymorphism of c4 genes in mice with t chromosomes

Mladen Golubić, Felipe Figueroa, Mario Tosi, Jan Klein

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Genomic DNA was isolated from 29 t strains and 4 congenic lines of mice, digested with restriction endonucleases, and hybridized with a probe representing the complement component 4 (C4) gene. All but one of the enzymes revealed restriction fragment length polymorphism in this sample of C4-related genes. Double digestion analysis suggested the presence of three C4 gene copies in some of the t chromosomes and two copies in others. The enzymes distinguished 16 different haplotypes among the 33 strains tested. Based on their restriction fragment length patterns, the t strains could be divided into four groups with strains in each group more closely related to each other with respect to their C4-region genes than strains belonging to different groups. At least three of these four groups represent different branches of the evolutionary tree constructed for the t chromosomes. The C4-related genes of the chromosomes are in strong linkage disequilibrium with the class II genes of the H-2 complex. Typing for the Ss and Slp allotypes of C4 has revealed the presence of the Ss1 phenotype in two t strains and of the Slpa phenotype in one strain.

Original languageEnglish (US)
Pages (from-to)247-256
Number of pages10
JournalImmunogenetics
Volume21
Issue number3
DOIs
StatePublished - Jan 1 1985

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Restriction Fragment Length Polymorphisms
Chromosomes
Genes
Congenic Mice
Gene Components
Complement C4
Phenotype
MHC Class II Genes
DNA Restriction Enzymes
Linkage Disequilibrium
Enzymes
Haplotypes
Digestion
DNA

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Golubić, Mladen ; Figueroa, Felipe ; Tosi, Mario ; Klein, Jan. / Restriction fragment length polymorphism of c4 genes in mice with t chromosomes. In: Immunogenetics. 1985 ; Vol. 21, No. 3. pp. 247-256.
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Golubić, M, Figueroa, F, Tosi, M & Klein, J 1985, 'Restriction fragment length polymorphism of c4 genes in mice with t chromosomes', Immunogenetics, vol. 21, no. 3, pp. 247-256. https://doi.org/10.1007/BF00375377

Restriction fragment length polymorphism of c4 genes in mice with t chromosomes. / Golubić, Mladen; Figueroa, Felipe; Tosi, Mario; Klein, Jan.

In: Immunogenetics, Vol. 21, No. 3, 01.01.1985, p. 247-256.

Research output: Contribution to journalArticle

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