Restriction site and genetic map of Cucurbita pepo chloroplast DNA

H. Lim, I. Gounaris, R. C. Hardison, C. D. Boyer

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

A detailed restriction map of squash chloroplast DNA (cpDNA) was constructed with five restriction endonuclease, SalI, PvuII, BglI, SacII, and PstI. The cleavage sites were mapped by sequential digestion of cpDNA using low-gelling temperature agarose. The restriction map shows that squash cpDNA is an approximately 153 kilobase (kb) circle with a large inverted repeat sequence of 23.3 kb, separated by a large (83.7 kb) and a small (22.7 kb) single copy region. Genes for a number of chloroplast polypeptides were localized on the map by hybridizing the cpDNA restriction fragments to heterologous gene-specific probes from tobacco, pea, tomato, maize, and spinach chloroplasts. The gene locations and organization of squash cpDNA are highly conserved and similar to chloroplast genomes of tomato, pepper, and Ginkgo.

Original languageEnglish (US)
Pages (from-to)273-275
Number of pages3
JournalCurrent Genetics
Volume18
Issue number3
DOIs
StatePublished - Oct 1 1990

All Science Journal Classification (ASJC) codes

  • Genetics

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