Sistemy restriktsii--modifikatsii II tipa v shtammakh Enterobacter cloacae.

Translated title of the contribution: Restriction system--type II modification in Enterobacter cloacae strains

E. V. Sineva, G. G. Zakharova, Z. E. Tarutina, A. N. Kravets, A. S. Solonin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Various clinical strains of Enterobacter cloacae have been examined for the presence of site-specific endonuclease activities. Restriction endonucleases of class II have been isolated from six strains. Recognition sequences for all of these endonucleases were determined and the cleavage sites were identified for two of them. The enzymes prove to be isoschizomers of EcoRII and PstI. Restriction endonucleases Ecl2zI and Ecl37kI recognise the nucleotide sequences CTGA/G and are true isoschizomers of PstI. The genes for Ecl54kI and Ecl57kI restriction-modification systems (isoschizomers of EcoRII) were found to be located on the IncN group plasmids, whereas the genes for Ecl2zI and Ecl99kI seem to be located on the chromosomes of host cells.

Original languageRussian
Pages (from-to)10-13
Number of pages4
JournalMolekuliarnaia genetika, mikrobiologiia i virusologiia
Issue number5
StatePublished - Sep 1 1993

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DNA Restriction-Modification Enzymes
Enterobacter cloacae
Endonucleases
DNA Restriction Enzymes
Genes
Plasmids
Chromosomes
Enzymes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Sineva, E. V., Zakharova, G. G., Tarutina, Z. E., Kravets, A. N., & Solonin, A. S. (1993). Sistemy restriktsii--modifikatsii II tipa v shtammakh Enterobacter cloacae. Molekuliarnaia genetika, mikrobiologiia i virusologiia, (5), 10-13.
Sineva, E. V. ; Zakharova, G. G. ; Tarutina, Z. E. ; Kravets, A. N. ; Solonin, A. S. / Sistemy restriktsii--modifikatsii II tipa v shtammakh Enterobacter cloacae. In: Molekuliarnaia genetika, mikrobiologiia i virusologiia. 1993 ; No. 5. pp. 10-13.
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Sineva, EV, Zakharova, GG, Tarutina, ZE, Kravets, AN & Solonin, AS 1993, 'Sistemy restriktsii--modifikatsii II tipa v shtammakh Enterobacter cloacae.', Molekuliarnaia genetika, mikrobiologiia i virusologiia, no. 5, pp. 10-13.

Sistemy restriktsii--modifikatsii II tipa v shtammakh Enterobacter cloacae. / Sineva, E. V.; Zakharova, G. G.; Tarutina, Z. E.; Kravets, A. N.; Solonin, A. S.

In: Molekuliarnaia genetika, mikrobiologiia i virusologiia, No. 5, 01.09.1993, p. 10-13.

Research output: Contribution to journalArticle

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AU - Sineva, E. V.

AU - Zakharova, G. G.

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AU - Kravets, A. N.

AU - Solonin, A. S.

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Sineva EV, Zakharova GG, Tarutina ZE, Kravets AN, Solonin AS. Sistemy restriktsii--modifikatsii II tipa v shtammakh Enterobacter cloacae. Molekuliarnaia genetika, mikrobiologiia i virusologiia. 1993 Sep 1;(5):10-13.