Ret 1, a cis-acting element of the rat opsin promoter, can direct gene expression in rod photoreceptors

Xiu Yu, Laurence Leconte, Julian A. Martinez, Colin Barnstable

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The Ret 1 element, located at -136 to -110 in the rat opsin promoter, binds developmentally regulated retinal nuclear proteins. A similar sequence is found upstream of opsin genes, from humans to Drosophila, as well as many other photoreceptor-specific genes. The function of the Ret 1 element was tested both in vitro and in two sets of transgenic mice. A mutated Ret 1 element did not bind retinal nuclear proteins in vitro. The same mutations in an otherwise normal 1.9-kb rat opsin promoter tailed to drive expression of a lacZ reporter gene in nine of 12 lines. In the three other lines, expression in photoreceptors was very faint. Four tandem copies of the Ret 1 element maintained the Ret 1 binding specificity in vitro and were able to direct expression of a lacZ transgene in photoreceptors of all nine mouse lines obtained. In two lines, expression was also detected in the ganglion cell layer and the ciliary epithelium. In three lines, a characteristic pattern of expression was found in the nervous system in addition to the normal retinal expression. These results indicate that Ret 1 can and is necessary to drive gene expression in rod photoreceptors. Furthermore, our results suggest that Ret 1-like elements may also be important in the developing nervous system.

Original languageEnglish (US)
Pages (from-to)2494-2504
Number of pages11
JournalJournal of Neurochemistry
Volume67
Issue number6
StatePublished - Dec 1996

Fingerprint

Opsins
Retinal Rod Photoreceptor Cells
Gene expression
Rats
Genes
Neurology
Nuclear Proteins
Gene Expression
Nervous System
Lac Operon
Transgenes
Reporter Genes
Ganglia
Transgenic Mice
Drosophila
Epithelium
Cells
Mutation
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

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title = "Ret 1, a cis-acting element of the rat opsin promoter, can direct gene expression in rod photoreceptors",
abstract = "The Ret 1 element, located at -136 to -110 in the rat opsin promoter, binds developmentally regulated retinal nuclear proteins. A similar sequence is found upstream of opsin genes, from humans to Drosophila, as well as many other photoreceptor-specific genes. The function of the Ret 1 element was tested both in vitro and in two sets of transgenic mice. A mutated Ret 1 element did not bind retinal nuclear proteins in vitro. The same mutations in an otherwise normal 1.9-kb rat opsin promoter tailed to drive expression of a lacZ reporter gene in nine of 12 lines. In the three other lines, expression in photoreceptors was very faint. Four tandem copies of the Ret 1 element maintained the Ret 1 binding specificity in vitro and were able to direct expression of a lacZ transgene in photoreceptors of all nine mouse lines obtained. In two lines, expression was also detected in the ganglion cell layer and the ciliary epithelium. In three lines, a characteristic pattern of expression was found in the nervous system in addition to the normal retinal expression. These results indicate that Ret 1 can and is necessary to drive gene expression in rod photoreceptors. Furthermore, our results suggest that Ret 1-like elements may also be important in the developing nervous system.",
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Ret 1, a cis-acting element of the rat opsin promoter, can direct gene expression in rod photoreceptors. / Yu, Xiu; Leconte, Laurence; Martinez, Julian A.; Barnstable, Colin.

In: Journal of Neurochemistry, Vol. 67, No. 6, 12.1996, p. 2494-2504.

Research output: Contribution to journalArticle

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