Retention of a cis Golgi protein requires polar residues on one face of a predicted α-helix in the transmembrane domain

C. E. Machamer, M. G. Grim, A. Esquela, S. W. Chung, M. Rolls, K. Ryan, A. M. Swift

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

The first membrane-spanning domain (m1) of the model cis Golgi protein M (formerly called E1) from the avian coronavirus infectious bronchitis virus is required for targeting to the Golgi complex. When inserted in place of the membrane-spanning domain of a plasma membrane protein (vesicular stomatitis virus G protein), the chimeric protein ('Gm1') is retained in the Golgi complex of transfected cells. To determine the precise features of the m1 domain responsible for Golgi targeting, we produced single amino acid substitutions in m1 and analyzed their effects on localization of Gm1. Expression at the plasma membrane was used as the criterion for loss of Golgi retention. Rates of oligosaccharide processing were used as a measure of rate and efficiency of transport through the Golgi complex. We identified four uncharged polar residues that are critical for Golgi retention of Gm1 (Asn465, Thr469, Thr476, and Gln480). These residues line one face of a predicted α-helix. Interestingly, when the m1 domain of the homologous M protein from mouse hepatitis virus is inserted into the G protein reporter, the chimeric protein is not efficiently retained in the Golgi complex, but transported to the cell surface. Although it possesses three of the four residues we identified as important in the avian m1 sequence, other residues in the membrane-spanning domain from the mouse protein must prevent efficient recognition of the polar face within the lipid bilayer of the cis Golgi.

Original languageEnglish (US)
Pages (from-to)695-704
Number of pages10
JournalMolecular biology of the cell
Volume4
Issue number7
DOIs
StatePublished - 1993

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Retention of a cis Golgi protein requires polar residues on one face of a predicted α-helix in the transmembrane domain'. Together they form a unique fingerprint.

  • Cite this