Retentive multipotency of adult dorsal root ganglia stem cells

Rabindra P. Singh, Ying Hua Cheng, Paul Nelson, Feng C. Zhou

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Preservation of neural stem cells (NSCs) in the adult peripheral nervous system (PNS) has recently been confirmed. However, it is not clear whether peripheral NSCs possess predestined, bona fide phenotypes or a response to innate developmental cues. In this study, we first demonstrated the longevity, multipotency, and high fidelity of sensory features of postmigrating adult dorsal root ganglia (aDRG) stem cells. Derived from aDRG and after 4-5 years in culture without dissociating, the aDRG NSCs were found capable of proliferation, expressing neuroepithelial, neuronal, and glial markers. Remarkably, these aDRG NSCs expressed sensory neuronal markers vesicular glutamate transporter2 (VGluT2 - glutamate terminals), transient receptor potential vanilloid1 (TrpV1 - capsaicin sensitive), phosphorylated 200 kDa neurofilaments (pNF200 - capsaicin insensitive, myelinated), and the serotonin transporter (5-HTT), which normally is transiently expressed in developing DRG. Furthermore, in response to neurotrophins, the aDRG NSCs enhanced TrpV1 expression upon exposure to nerve growth factor (NGF), but not to brain-derived neurotrophic factor (BDNF). On the contrary, BDNF increased the expression of NeuN. Third, the characterization of aDRG NSCs was demonstrated by transplantation of red fluorescent-expressing aDRG NSCs into injured spinal cord. These cells expressed nestin, Hu, and β-III-tubulin (immature neuronal markers), GFAP (astrocyte marker) as well as sensory neural marker TrpV1 (capsaicin sensitive) and pNF200 (mature, capsaicin insensitive, myelinated). Our results demonstrated that the postmigrating neural crest adult DRG stem cells not only preserved their multipotency but also were retentive in sensory potency despite the age and long-term ex vivo status.

Original languageEnglish (US)
Pages (from-to)55-68
Number of pages14
JournalCell Transplantation
Volume18
Issue number1
DOIs
StatePublished - Jun 16 2009

Fingerprint

Neural Stem Cells
Spinal Ganglia
Stem cells
Stem Cells
Capsaicin
Diagnosis-Related Groups
Brain-Derived Neurotrophic Factor
Glutamic Acid
Brain
Nestin
Serotonin Plasma Membrane Transport Proteins
Adult Stem Cells
Neural Crest
Peripheral Nervous System
Nerve Growth Factors
Nerve Growth Factor
Tubulin
Neuroglia
Astrocytes
Cues

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

Cite this

Singh, Rabindra P. ; Cheng, Ying Hua ; Nelson, Paul ; Zhou, Feng C. / Retentive multipotency of adult dorsal root ganglia stem cells. In: Cell Transplantation. 2009 ; Vol. 18, No. 1. pp. 55-68.
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Retentive multipotency of adult dorsal root ganglia stem cells. / Singh, Rabindra P.; Cheng, Ying Hua; Nelson, Paul; Zhou, Feng C.

In: Cell Transplantation, Vol. 18, No. 1, 16.06.2009, p. 55-68.

Research output: Contribution to journalArticle

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