Retinal ganglion cells express a cGMP-gated cation conductance activatable by nitric oxide donors

Iqbal Ahmad, Trese Leinders-Zufall, Jeffery D. Kocsis, Gordon M. Shepherd, Frank Zufall, Colin Barnstable

Research output: Contribution to journalArticle

216 Citations (Scopus)

Abstract

We have identified a putative cGMP-gated cation conductance in rat retinal ganglion cells. Both in situ hybridization and polymerase chain reaction amplification detected transcripts in ganglion cells that were highly homologous to the cGMP-gated cation channel expressed in rod photoreceptors. Whole-cell patch-clamp recordings detected a current stimulated by cGMP due to activation of nonselective cation channels. This current had a reversal potential near 0 mV, showed some outward rectification, and could be blocked by Cd2+. The current could also be activated by a phosphodiesterase inhibitor and the nitric oxide donors sodium nitroprusside and S-nitrosocysteine. We propose that nitric oxide released from an identified subpopulation of amacrine cells may activate this channel to modulate ganglion cell activity.

Original languageEnglish (US)
Pages (from-to)155-165
Number of pages11
JournalNeuron
Volume12
Issue number1
DOIs
StatePublished - Jan 1 1994

Fingerprint

Nitric Oxide Donors
Retinal Ganglion Cells
Cations
Ganglia
Retinal Rod Photoreceptor Cells
Amacrine Cells
Phosphodiesterase Inhibitors
Nitroprusside
In Situ Hybridization
Nitric Oxide
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Ahmad, Iqbal ; Leinders-Zufall, Trese ; Kocsis, Jeffery D. ; Shepherd, Gordon M. ; Zufall, Frank ; Barnstable, Colin. / Retinal ganglion cells express a cGMP-gated cation conductance activatable by nitric oxide donors. In: Neuron. 1994 ; Vol. 12, No. 1. pp. 155-165.
@article{2d8ea82cff0842b19fb140d8b854f4e1,
title = "Retinal ganglion cells express a cGMP-gated cation conductance activatable by nitric oxide donors",
abstract = "We have identified a putative cGMP-gated cation conductance in rat retinal ganglion cells. Both in situ hybridization and polymerase chain reaction amplification detected transcripts in ganglion cells that were highly homologous to the cGMP-gated cation channel expressed in rod photoreceptors. Whole-cell patch-clamp recordings detected a current stimulated by cGMP due to activation of nonselective cation channels. This current had a reversal potential near 0 mV, showed some outward rectification, and could be blocked by Cd2+. The current could also be activated by a phosphodiesterase inhibitor and the nitric oxide donors sodium nitroprusside and S-nitrosocysteine. We propose that nitric oxide released from an identified subpopulation of amacrine cells may activate this channel to modulate ganglion cell activity.",
author = "Iqbal Ahmad and Trese Leinders-Zufall and Kocsis, {Jeffery D.} and Shepherd, {Gordon M.} and Frank Zufall and Colin Barnstable",
year = "1994",
month = "1",
day = "1",
doi = "10.1016/0896-6273(94)90160-0",
language = "English (US)",
volume = "12",
pages = "155--165",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "1",

}

Retinal ganglion cells express a cGMP-gated cation conductance activatable by nitric oxide donors. / Ahmad, Iqbal; Leinders-Zufall, Trese; Kocsis, Jeffery D.; Shepherd, Gordon M.; Zufall, Frank; Barnstable, Colin.

In: Neuron, Vol. 12, No. 1, 01.01.1994, p. 155-165.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Retinal ganglion cells express a cGMP-gated cation conductance activatable by nitric oxide donors

AU - Ahmad, Iqbal

AU - Leinders-Zufall, Trese

AU - Kocsis, Jeffery D.

AU - Shepherd, Gordon M.

AU - Zufall, Frank

AU - Barnstable, Colin

PY - 1994/1/1

Y1 - 1994/1/1

N2 - We have identified a putative cGMP-gated cation conductance in rat retinal ganglion cells. Both in situ hybridization and polymerase chain reaction amplification detected transcripts in ganglion cells that were highly homologous to the cGMP-gated cation channel expressed in rod photoreceptors. Whole-cell patch-clamp recordings detected a current stimulated by cGMP due to activation of nonselective cation channels. This current had a reversal potential near 0 mV, showed some outward rectification, and could be blocked by Cd2+. The current could also be activated by a phosphodiesterase inhibitor and the nitric oxide donors sodium nitroprusside and S-nitrosocysteine. We propose that nitric oxide released from an identified subpopulation of amacrine cells may activate this channel to modulate ganglion cell activity.

AB - We have identified a putative cGMP-gated cation conductance in rat retinal ganglion cells. Both in situ hybridization and polymerase chain reaction amplification detected transcripts in ganglion cells that were highly homologous to the cGMP-gated cation channel expressed in rod photoreceptors. Whole-cell patch-clamp recordings detected a current stimulated by cGMP due to activation of nonselective cation channels. This current had a reversal potential near 0 mV, showed some outward rectification, and could be blocked by Cd2+. The current could also be activated by a phosphodiesterase inhibitor and the nitric oxide donors sodium nitroprusside and S-nitrosocysteine. We propose that nitric oxide released from an identified subpopulation of amacrine cells may activate this channel to modulate ganglion cell activity.

UR - http://www.scopus.com/inward/record.url?scp=0028178804&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028178804&partnerID=8YFLogxK

U2 - 10.1016/0896-6273(94)90160-0

DO - 10.1016/0896-6273(94)90160-0

M3 - Article

C2 - 7507337

AN - SCOPUS:0028178804

VL - 12

SP - 155

EP - 165

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 1

ER -