Retinal Thy-1 expression during development

Catherine Jui Ling Liu, Neena Chaturvedi, Colin Barnstable, Evan B. Dreyer

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Purpose. To evaluate the developmental expression of Thy-1 in the retina. Thy-1, the most abundant mammalian neuronal surface glycoprotein, is likely to play a significant role in retinal development. In the mammalian retina, it is found predominantly, if not exclusively, on retinal ganglion cells. Methods. Rat retinae of various ages were stained immunohistochemically for Thy-1 with 2G12, a monoclonal Thy-1 antibody. Sections were analyzed digitally to quantify bound antibody. Using semiquantitative reverse transcription-polymerase chain reaction (RT-PCR), the expression of Thy-1 protein was compared with the levels of mRNA detected. Results. Thy-1- dependent fluorescence was detected in rat retinae from birth, albeit at low levels. Thy-1 labeling was localized predominantly to the ganglion cell layer. Minimal, fine patterns of linear and reticular fluorescence were noted in the inner nuclear layer. Thy-1 levels reached a maximal level at approximately postnatal day 14. RT-PCR measurements showed a similar time course for the increase in Thy-1 expression. Conclusion. The Thy-1 antigen is present in the inner retina at birth. Its level increases steadily after birth and peaks during the second week of life. Thy-1 expression is approximately coterminous with synaptogenesis of the inner plexiform layer and may play a role in synaptogenesis of the inner retina or in other developmental milestones in the formation of the visual system.

Original languageEnglish (US)
Pages (from-to)1469-1473
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number7
StatePublished - Jun 1996

Fingerprint

Retina
Parturition
Reverse Transcription
Fluorescence
Thy-1 Antigens
Polymerase Chain Reaction
Retinal Ganglion Cells
Antibodies
Membrane Glycoproteins
Ganglia
Messenger RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Liu, C. J. L., Chaturvedi, N., Barnstable, C., & Dreyer, E. B. (1996). Retinal Thy-1 expression during development. Investigative Ophthalmology and Visual Science, 37(7), 1469-1473.
Liu, Catherine Jui Ling ; Chaturvedi, Neena ; Barnstable, Colin ; Dreyer, Evan B. / Retinal Thy-1 expression during development. In: Investigative Ophthalmology and Visual Science. 1996 ; Vol. 37, No. 7. pp. 1469-1473.
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Liu, CJL, Chaturvedi, N, Barnstable, C & Dreyer, EB 1996, 'Retinal Thy-1 expression during development', Investigative Ophthalmology and Visual Science, vol. 37, no. 7, pp. 1469-1473.

Retinal Thy-1 expression during development. / Liu, Catherine Jui Ling; Chaturvedi, Neena; Barnstable, Colin; Dreyer, Evan B.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 7, 06.1996, p. 1469-1473.

Research output: Contribution to journalArticle

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AU - Liu, Catherine Jui Ling

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AU - Barnstable, Colin

AU - Dreyer, Evan B.

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N2 - Purpose. To evaluate the developmental expression of Thy-1 in the retina. Thy-1, the most abundant mammalian neuronal surface glycoprotein, is likely to play a significant role in retinal development. In the mammalian retina, it is found predominantly, if not exclusively, on retinal ganglion cells. Methods. Rat retinae of various ages were stained immunohistochemically for Thy-1 with 2G12, a monoclonal Thy-1 antibody. Sections were analyzed digitally to quantify bound antibody. Using semiquantitative reverse transcription-polymerase chain reaction (RT-PCR), the expression of Thy-1 protein was compared with the levels of mRNA detected. Results. Thy-1- dependent fluorescence was detected in rat retinae from birth, albeit at low levels. Thy-1 labeling was localized predominantly to the ganglion cell layer. Minimal, fine patterns of linear and reticular fluorescence were noted in the inner nuclear layer. Thy-1 levels reached a maximal level at approximately postnatal day 14. RT-PCR measurements showed a similar time course for the increase in Thy-1 expression. Conclusion. The Thy-1 antigen is present in the inner retina at birth. Its level increases steadily after birth and peaks during the second week of life. Thy-1 expression is approximately coterminous with synaptogenesis of the inner plexiform layer and may play a role in synaptogenesis of the inner retina or in other developmental milestones in the formation of the visual system.

AB - Purpose. To evaluate the developmental expression of Thy-1 in the retina. Thy-1, the most abundant mammalian neuronal surface glycoprotein, is likely to play a significant role in retinal development. In the mammalian retina, it is found predominantly, if not exclusively, on retinal ganglion cells. Methods. Rat retinae of various ages were stained immunohistochemically for Thy-1 with 2G12, a monoclonal Thy-1 antibody. Sections were analyzed digitally to quantify bound antibody. Using semiquantitative reverse transcription-polymerase chain reaction (RT-PCR), the expression of Thy-1 protein was compared with the levels of mRNA detected. Results. Thy-1- dependent fluorescence was detected in rat retinae from birth, albeit at low levels. Thy-1 labeling was localized predominantly to the ganglion cell layer. Minimal, fine patterns of linear and reticular fluorescence were noted in the inner nuclear layer. Thy-1 levels reached a maximal level at approximately postnatal day 14. RT-PCR measurements showed a similar time course for the increase in Thy-1 expression. Conclusion. The Thy-1 antigen is present in the inner retina at birth. Its level increases steadily after birth and peaks during the second week of life. Thy-1 expression is approximately coterminous with synaptogenesis of the inner plexiform layer and may play a role in synaptogenesis of the inner retina or in other developmental milestones in the formation of the visual system.

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