Retinoic acid blockade of imidazole-induced tyrosinase expression in B16 melanoma cultures: Similar effects of the active retinoid and triiodothyronine

Terry J. Smith, James T. Warren, Ellis L. Kline

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The effect of retinoic acid on the induction of tyrosinase (EC 1:14.18.1) by imidazole was determined in cultured B16 C3 melanoma cells. Retinoic acid could block the induction of enzyme activity within 3 hours of addition to the culture medium at a physiological concentration (10nM). The blockade was similar to that of 3,3′,5-L-triiodothyronine (T3) already reported. mRNA hybridizable to a tyrosinase DNA probe was induced by imidazole while retinoic acid and T3 blocked that increase. These observations suggest that retinoic acid can mimic the action of T3 in B16 melanoma cells in culture.

Original languageEnglish (US)
Pages (from-to)288-293
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume162
Issue number1
DOIs
StatePublished - Jul 14 1989

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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