Retinol-binding protein gene expression in cyclic and pregnant endometrium of pigs, sheep, and cattle

J. P. Harney, Troy Ott, R. D. Geisert, F. W. Bazer

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Retinol-binding protein (RBP) is a major secretory product of conceptuses and endometrium of the large domestic animals. The present study examined the abundance of RBP mRNA in cyclic and early pregnant endometrium of pigs, sheep, and cattle and confirmed the presence of RBP mRNA in periimplantation conceptuses of the large domestic animals. Northern blot analysis, using porcine liver RBP cDNA as the probe, detected a 1.0-1.1-kb transcript in conceptuses and endometrium collected during the periimplantation period of pregnancy in pigs (Day 15), sheep (Day 16), and cows (Day 18). Slot-blot analysis of RBP mRNA in endometrium of pigs, sheep, and cows indicated differential RBP gene expression across days and statuses in pigs and sheep and across days alone in cows. In pigs, RBP gene expression was low to undetectable at Days 0, 5, and 10 of the estrous cycle and Day 10 of pregnancy. Levels of RBP mRNA increased (p < 0.06) from Days 10 to 12 and further (p < 0.001) to Day 15 across both statuses. At Day 18, RBP mRNA levels decreased (p < 0.01) in cyclic pigs but remained elevated in endometrium of pregnant pigs. In sheep, levels of RBP mRNA increased between Days 10 and 12 and 14 in both cyclic and pregnant ewes: however, between Days 14 and 16, RBP mRNA levels remained elevated in cyclic endometrium but decreased (p < 0.01) in endometrium of pregnant ewes. In a second experiment, RBP mRNA levels increased (p < 0.01) between Days 0-5 and Days 13-15 of the estrous cycle. RBP gene expression was similar in pregnant and cyclic ewes on Days 11 and 13, then increased in cyclic (p = 0.07) but not pregnant ewes, supporting results from experiment 1. Levels of RBP mRNA increased in endometrium of both cyclic and pregnant cows between Days 10, 15, and 18; then cows, like sheep but unlike pigs, exhibited RBP mRNA at estrus (Day 0) and metestrus (Day 5). Results suggest differential RBP gene expression in endometrium of large domestic animals and support an important role for the vitamin A transport protein in uterine and conceptus physiology of early pregnancy.

Original languageEnglish (US)
Pages (from-to)1066-1073
Number of pages8
JournalBiology of Reproduction
Volume49
Issue number5
DOIs
StatePublished - Nov 1 1993

Fingerprint

Retinol-Binding Proteins
Endometrium
Sheep
Swine
Gene Expression
Messenger RNA
Domestic Animals
Estrous Cycle
Pregnancy
Metestrus
Estrus

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Harney, J. P. ; Ott, Troy ; Geisert, R. D. ; Bazer, F. W. / Retinol-binding protein gene expression in cyclic and pregnant endometrium of pigs, sheep, and cattle. In: Biology of Reproduction. 1993 ; Vol. 49, No. 5. pp. 1066-1073.
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abstract = "Retinol-binding protein (RBP) is a major secretory product of conceptuses and endometrium of the large domestic animals. The present study examined the abundance of RBP mRNA in cyclic and early pregnant endometrium of pigs, sheep, and cattle and confirmed the presence of RBP mRNA in periimplantation conceptuses of the large domestic animals. Northern blot analysis, using porcine liver RBP cDNA as the probe, detected a 1.0-1.1-kb transcript in conceptuses and endometrium collected during the periimplantation period of pregnancy in pigs (Day 15), sheep (Day 16), and cows (Day 18). Slot-blot analysis of RBP mRNA in endometrium of pigs, sheep, and cows indicated differential RBP gene expression across days and statuses in pigs and sheep and across days alone in cows. In pigs, RBP gene expression was low to undetectable at Days 0, 5, and 10 of the estrous cycle and Day 10 of pregnancy. Levels of RBP mRNA increased (p < 0.06) from Days 10 to 12 and further (p < 0.001) to Day 15 across both statuses. At Day 18, RBP mRNA levels decreased (p < 0.01) in cyclic pigs but remained elevated in endometrium of pregnant pigs. In sheep, levels of RBP mRNA increased between Days 10 and 12 and 14 in both cyclic and pregnant ewes: however, between Days 14 and 16, RBP mRNA levels remained elevated in cyclic endometrium but decreased (p < 0.01) in endometrium of pregnant ewes. In a second experiment, RBP mRNA levels increased (p < 0.01) between Days 0-5 and Days 13-15 of the estrous cycle. RBP gene expression was similar in pregnant and cyclic ewes on Days 11 and 13, then increased in cyclic (p = 0.07) but not pregnant ewes, supporting results from experiment 1. Levels of RBP mRNA increased in endometrium of both cyclic and pregnant cows between Days 10, 15, and 18; then cows, like sheep but unlike pigs, exhibited RBP mRNA at estrus (Day 0) and metestrus (Day 5). Results suggest differential RBP gene expression in endometrium of large domestic animals and support an important role for the vitamin A transport protein in uterine and conceptus physiology of early pregnancy.",
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Retinol-binding protein gene expression in cyclic and pregnant endometrium of pigs, sheep, and cattle. / Harney, J. P.; Ott, Troy; Geisert, R. D.; Bazer, F. W.

In: Biology of Reproduction, Vol. 49, No. 5, 01.11.1993, p. 1066-1073.

Research output: Contribution to journalArticle

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T1 - Retinol-binding protein gene expression in cyclic and pregnant endometrium of pigs, sheep, and cattle

AU - Harney, J. P.

AU - Ott, Troy

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N2 - Retinol-binding protein (RBP) is a major secretory product of conceptuses and endometrium of the large domestic animals. The present study examined the abundance of RBP mRNA in cyclic and early pregnant endometrium of pigs, sheep, and cattle and confirmed the presence of RBP mRNA in periimplantation conceptuses of the large domestic animals. Northern blot analysis, using porcine liver RBP cDNA as the probe, detected a 1.0-1.1-kb transcript in conceptuses and endometrium collected during the periimplantation period of pregnancy in pigs (Day 15), sheep (Day 16), and cows (Day 18). Slot-blot analysis of RBP mRNA in endometrium of pigs, sheep, and cows indicated differential RBP gene expression across days and statuses in pigs and sheep and across days alone in cows. In pigs, RBP gene expression was low to undetectable at Days 0, 5, and 10 of the estrous cycle and Day 10 of pregnancy. Levels of RBP mRNA increased (p < 0.06) from Days 10 to 12 and further (p < 0.001) to Day 15 across both statuses. At Day 18, RBP mRNA levels decreased (p < 0.01) in cyclic pigs but remained elevated in endometrium of pregnant pigs. In sheep, levels of RBP mRNA increased between Days 10 and 12 and 14 in both cyclic and pregnant ewes: however, between Days 14 and 16, RBP mRNA levels remained elevated in cyclic endometrium but decreased (p < 0.01) in endometrium of pregnant ewes. In a second experiment, RBP mRNA levels increased (p < 0.01) between Days 0-5 and Days 13-15 of the estrous cycle. RBP gene expression was similar in pregnant and cyclic ewes on Days 11 and 13, then increased in cyclic (p = 0.07) but not pregnant ewes, supporting results from experiment 1. Levels of RBP mRNA increased in endometrium of both cyclic and pregnant cows between Days 10, 15, and 18; then cows, like sheep but unlike pigs, exhibited RBP mRNA at estrus (Day 0) and metestrus (Day 5). Results suggest differential RBP gene expression in endometrium of large domestic animals and support an important role for the vitamin A transport protein in uterine and conceptus physiology of early pregnancy.

AB - Retinol-binding protein (RBP) is a major secretory product of conceptuses and endometrium of the large domestic animals. The present study examined the abundance of RBP mRNA in cyclic and early pregnant endometrium of pigs, sheep, and cattle and confirmed the presence of RBP mRNA in periimplantation conceptuses of the large domestic animals. Northern blot analysis, using porcine liver RBP cDNA as the probe, detected a 1.0-1.1-kb transcript in conceptuses and endometrium collected during the periimplantation period of pregnancy in pigs (Day 15), sheep (Day 16), and cows (Day 18). Slot-blot analysis of RBP mRNA in endometrium of pigs, sheep, and cows indicated differential RBP gene expression across days and statuses in pigs and sheep and across days alone in cows. In pigs, RBP gene expression was low to undetectable at Days 0, 5, and 10 of the estrous cycle and Day 10 of pregnancy. Levels of RBP mRNA increased (p < 0.06) from Days 10 to 12 and further (p < 0.001) to Day 15 across both statuses. At Day 18, RBP mRNA levels decreased (p < 0.01) in cyclic pigs but remained elevated in endometrium of pregnant pigs. In sheep, levels of RBP mRNA increased between Days 10 and 12 and 14 in both cyclic and pregnant ewes: however, between Days 14 and 16, RBP mRNA levels remained elevated in cyclic endometrium but decreased (p < 0.01) in endometrium of pregnant ewes. In a second experiment, RBP mRNA levels increased (p < 0.01) between Days 0-5 and Days 13-15 of the estrous cycle. RBP gene expression was similar in pregnant and cyclic ewes on Days 11 and 13, then increased in cyclic (p = 0.07) but not pregnant ewes, supporting results from experiment 1. Levels of RBP mRNA increased in endometrium of both cyclic and pregnant cows between Days 10, 15, and 18; then cows, like sheep but unlike pigs, exhibited RBP mRNA at estrus (Day 0) and metestrus (Day 5). Results suggest differential RBP gene expression in endometrium of large domestic animals and support an important role for the vitamin A transport protein in uterine and conceptus physiology of early pregnancy.

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