Reusable platforms for high-throughput on-chip temperature gradient assays

Hanbin Mao, Matthew A. Holden, Min You, Paul S. Cremer

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

This paper describes a reusable platform that can apply a linear temperature gradient to a lab-on-a-chip device. When a planar microfluidic device with a series of microchannels is placed on top of the platform with the channels perpendicular to the gradient, each channel is held at a discrete temperature. This allows temperature-dependent data for chemical or biochemical species flowed into the device to be obtained in a concurrent fashion. As a demonstration, a melting curve for dsDNA is performed by collecting all the data simultaneously. The gradient is stable enough to easily distinguish between 30-mers where the complement strand contains a single C-A mismatch or a single T- G mismatch or is a perfect match. On the other hand, a temperature gradient can be formed parallel to the direction of flow of the microchannels. This allows the temperature in each channel to vary continuously as the liquid flows downstream. If each microchannel in the array contains a distinct pH value, ionic strength, species concentration, or chemical composition, then a high-throughput two-variable experiment can be performed. We demonstrate this mode of data collection by measuring the fluorescence yield of fluorescein dye molecules in aqueous solution simultaneously as a function of concentration and temperature.

Original languageEnglish (US)
Pages (from-to)5071-5075
Number of pages5
JournalAnalytical Chemistry
Volume74
Issue number19
DOIs
StatePublished - Oct 1 2002

Fingerprint

Thermal gradients
Assays
Microchannels
Throughput
Lab-on-a-chip
Temperature
Ionic strength
Fluorescein
Microfluidics
Melting
Coloring Agents
Demonstrations
Fluorescence
Molecules
Liquids
Chemical analysis
Experiments

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Mao, Hanbin ; Holden, Matthew A. ; You, Min ; Cremer, Paul S. / Reusable platforms for high-throughput on-chip temperature gradient assays. In: Analytical Chemistry. 2002 ; Vol. 74, No. 19. pp. 5071-5075.
@article{12a9f982d8ca49699762049c1a47fb4a,
title = "Reusable platforms for high-throughput on-chip temperature gradient assays",
abstract = "This paper describes a reusable platform that can apply a linear temperature gradient to a lab-on-a-chip device. When a planar microfluidic device with a series of microchannels is placed on top of the platform with the channels perpendicular to the gradient, each channel is held at a discrete temperature. This allows temperature-dependent data for chemical or biochemical species flowed into the device to be obtained in a concurrent fashion. As a demonstration, a melting curve for dsDNA is performed by collecting all the data simultaneously. The gradient is stable enough to easily distinguish between 30-mers where the complement strand contains a single C-A mismatch or a single T- G mismatch or is a perfect match. On the other hand, a temperature gradient can be formed parallel to the direction of flow of the microchannels. This allows the temperature in each channel to vary continuously as the liquid flows downstream. If each microchannel in the array contains a distinct pH value, ionic strength, species concentration, or chemical composition, then a high-throughput two-variable experiment can be performed. We demonstrate this mode of data collection by measuring the fluorescence yield of fluorescein dye molecules in aqueous solution simultaneously as a function of concentration and temperature.",
author = "Hanbin Mao and Holden, {Matthew A.} and Min You and Cremer, {Paul S.}",
year = "2002",
month = "10",
day = "1",
doi = "10.1021/ac025851z",
language = "English (US)",
volume = "74",
pages = "5071--5075",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "19",

}

Reusable platforms for high-throughput on-chip temperature gradient assays. / Mao, Hanbin; Holden, Matthew A.; You, Min; Cremer, Paul S.

In: Analytical Chemistry, Vol. 74, No. 19, 01.10.2002, p. 5071-5075.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Reusable platforms for high-throughput on-chip temperature gradient assays

AU - Mao, Hanbin

AU - Holden, Matthew A.

AU - You, Min

AU - Cremer, Paul S.

PY - 2002/10/1

Y1 - 2002/10/1

N2 - This paper describes a reusable platform that can apply a linear temperature gradient to a lab-on-a-chip device. When a planar microfluidic device with a series of microchannels is placed on top of the platform with the channels perpendicular to the gradient, each channel is held at a discrete temperature. This allows temperature-dependent data for chemical or biochemical species flowed into the device to be obtained in a concurrent fashion. As a demonstration, a melting curve for dsDNA is performed by collecting all the data simultaneously. The gradient is stable enough to easily distinguish between 30-mers where the complement strand contains a single C-A mismatch or a single T- G mismatch or is a perfect match. On the other hand, a temperature gradient can be formed parallel to the direction of flow of the microchannels. This allows the temperature in each channel to vary continuously as the liquid flows downstream. If each microchannel in the array contains a distinct pH value, ionic strength, species concentration, or chemical composition, then a high-throughput two-variable experiment can be performed. We demonstrate this mode of data collection by measuring the fluorescence yield of fluorescein dye molecules in aqueous solution simultaneously as a function of concentration and temperature.

AB - This paper describes a reusable platform that can apply a linear temperature gradient to a lab-on-a-chip device. When a planar microfluidic device with a series of microchannels is placed on top of the platform with the channels perpendicular to the gradient, each channel is held at a discrete temperature. This allows temperature-dependent data for chemical or biochemical species flowed into the device to be obtained in a concurrent fashion. As a demonstration, a melting curve for dsDNA is performed by collecting all the data simultaneously. The gradient is stable enough to easily distinguish between 30-mers where the complement strand contains a single C-A mismatch or a single T- G mismatch or is a perfect match. On the other hand, a temperature gradient can be formed parallel to the direction of flow of the microchannels. This allows the temperature in each channel to vary continuously as the liquid flows downstream. If each microchannel in the array contains a distinct pH value, ionic strength, species concentration, or chemical composition, then a high-throughput two-variable experiment can be performed. We demonstrate this mode of data collection by measuring the fluorescence yield of fluorescein dye molecules in aqueous solution simultaneously as a function of concentration and temperature.

UR - http://www.scopus.com/inward/record.url?scp=0036787662&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036787662&partnerID=8YFLogxK

U2 - 10.1021/ac025851z

DO - 10.1021/ac025851z

M3 - Article

VL - 74

SP - 5071

EP - 5075

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 19

ER -