Reversal of cholinesterase inhibition and clinical signs and the postmortem findings in mice after intraperitoneal administration of anatoxin-a(s), paraoxon or pyridostigmine

W. O. Cook, A. M. Dahlem, K. S. Harlin, Val Richard Beasley, S. B. Hooser, W. M. Haschek, W. W. Carmicheal

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The reversibility of inhibition of plasma, red blood cell (RBC), and diaphragm cholinesterase (ChE) and clinical signs in mice given anatoxin-a(s) [antx-a(s)], a ChE inhibitor from Anabaena flos-aquae NRC-525-17, were characterized and compared with the effects of 2 known ChE inhibitors, the organophosphorus compound paraoxon and the carbamate pyridostigmine bromide. To follow recovery of ChE activity, mice were given either a control solution or an LD40 dose of one of the toxicants ip and killed at time points up to 8 d postdosing. After dosing, mice were monitored for diarrhea, fasciculations, respiratory difficulty, salivation, and tremors. In general, clinical signs in mice given antx-a(s) persisted longer than in mice given pyridostigmine and were more similar in duration to the clinical signs in mice given paraoxon. Histologic lesions were not detected in tissues of mice killed after administration of antx-a(s). Anatoxin-a(s) inhibited plasma and diaphragm ChE for greater than 1 but less than 2 d and RBC ChE for 8 d. The time required for recovery from Antx-a(s)-induced inhibition of ChE in plasma, RBC, and diaphragm was similar to or longer than that with paraoxon and longer than that with pyridostigmine. Based on the duration of antx-a(s) induced clinical signs and ChE inhibition in mice, antx-a(s) appears to be an in vivo irreversible inhibitor of ChE.

Original languageEnglish (US)
Pages (from-to)1-4
Number of pages4
JournalVeterinary and Human Toxicology
Volume33
Issue number1
StatePublished - 1991

Fingerprint

anatoxins
Pyridostigmine Bromide
Paraoxon
paraoxon
cholinesterase
Cholinesterases
intraperitoneal injection
Cholinesterase Inhibitors
mice
Diaphragms
Blood
cholinesterase inhibitors
Plasmas
diaphragm
Diaphragm
Organophosphorus Compounds
Recovery
erythrocytes
Carbamates
Erythrocytes

All Science Journal Classification (ASJC) codes

  • veterinary(all)
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

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title = "Reversal of cholinesterase inhibition and clinical signs and the postmortem findings in mice after intraperitoneal administration of anatoxin-a(s), paraoxon or pyridostigmine",
abstract = "The reversibility of inhibition of plasma, red blood cell (RBC), and diaphragm cholinesterase (ChE) and clinical signs in mice given anatoxin-a(s) [antx-a(s)], a ChE inhibitor from Anabaena flos-aquae NRC-525-17, were characterized and compared with the effects of 2 known ChE inhibitors, the organophosphorus compound paraoxon and the carbamate pyridostigmine bromide. To follow recovery of ChE activity, mice were given either a control solution or an LD40 dose of one of the toxicants ip and killed at time points up to 8 d postdosing. After dosing, mice were monitored for diarrhea, fasciculations, respiratory difficulty, salivation, and tremors. In general, clinical signs in mice given antx-a(s) persisted longer than in mice given pyridostigmine and were more similar in duration to the clinical signs in mice given paraoxon. Histologic lesions were not detected in tissues of mice killed after administration of antx-a(s). Anatoxin-a(s) inhibited plasma and diaphragm ChE for greater than 1 but less than 2 d and RBC ChE for 8 d. The time required for recovery from Antx-a(s)-induced inhibition of ChE in plasma, RBC, and diaphragm was similar to or longer than that with paraoxon and longer than that with pyridostigmine. Based on the duration of antx-a(s) induced clinical signs and ChE inhibition in mice, antx-a(s) appears to be an in vivo irreversible inhibitor of ChE.",
author = "Cook, {W. O.} and Dahlem, {A. M.} and Harlin, {K. S.} and Beasley, {Val Richard} and Hooser, {S. B.} and Haschek, {W. M.} and Carmicheal, {W. W.}",
year = "1991",
language = "English (US)",
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Reversal of cholinesterase inhibition and clinical signs and the postmortem findings in mice after intraperitoneal administration of anatoxin-a(s), paraoxon or pyridostigmine. / Cook, W. O.; Dahlem, A. M.; Harlin, K. S.; Beasley, Val Richard; Hooser, S. B.; Haschek, W. M.; Carmicheal, W. W.

In: Veterinary and Human Toxicology, Vol. 33, No. 1, 1991, p. 1-4.

Research output: Contribution to journalArticle

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T1 - Reversal of cholinesterase inhibition and clinical signs and the postmortem findings in mice after intraperitoneal administration of anatoxin-a(s), paraoxon or pyridostigmine

AU - Cook, W. O.

AU - Dahlem, A. M.

AU - Harlin, K. S.

AU - Beasley, Val Richard

AU - Hooser, S. B.

AU - Haschek, W. M.

AU - Carmicheal, W. W.

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