Reverse breeding: A novel breeding approach based on engineered meiosis

Rob Dirks, Kees Van Dun, C. Bastiaan De Snoo, Mark Van Den Berg, Cilia L.C. Lelivelt, William Voermans, Leo Woudenberg, Jack P.C. De Wit, Kees Reinink, Johan W. Schut, Eveline Van Der Zeeuw, Aat Vogelaar, Gerald Freymark, Evert W. Gutteling, Marina N. Keppel, Paul Van Drongelen, Matthieu Kieny, Philippe Ellul, Alisher Touraev, Hong MaHans De Jong, Erik Wijnker

Research output: Contribution to journalReview article

49 Citations (Scopus)

Abstract

Reverse breeding (RB) is a novel plant breeding technique designed to directly produce parental lines for any heterozygous plant, one of the most sought after goals in plant breeding. RB generates perfectly complementing homozygous parental lines through engineered meiosis. The method is based on reducing genetic recombination in the selected heterozygote by eliminating meiotic crossing over. Male or female spores obtained from such plants contain combinations of non-recombinant parental chromosomes which can be cultured in vitro to generate homozygous doubled haploid plants (DHs). From these DHs, complementary parents can be selected and used to reconstitute the heterozygote in perpetuity. Since the fixation of unknown heterozygous genotypes is impossible in traditional plant breeding, RB could fundamentally change future plant breeding. In this review, we discuss various other applications of RB, including breeding per chromosome.

Original languageEnglish (US)
Pages (from-to)837-845
Number of pages9
JournalPlant Biotechnology Journal
Volume7
Issue number9
DOIs
StatePublished - Dec 1 2009

Fingerprint

Meiosis
meiosis
Breeding
plant breeding
breeding
heterozygosity
Heterozygote
Chromosomes
chromosomes
genetic recombination
breeding methods
crossing over
Haploidy
doubled haploids
Spores
Genetic Recombination
spores
Genotype
Plant Breeding

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Dirks, R., Van Dun, K., De Snoo, C. B., Van Den Berg, M., Lelivelt, C. L. C., Voermans, W., ... Wijnker, E. (2009). Reverse breeding: A novel breeding approach based on engineered meiosis. Plant Biotechnology Journal, 7(9), 837-845. https://doi.org/10.1111/j.1467-7652.2009.00450.x
Dirks, Rob ; Van Dun, Kees ; De Snoo, C. Bastiaan ; Van Den Berg, Mark ; Lelivelt, Cilia L.C. ; Voermans, William ; Woudenberg, Leo ; De Wit, Jack P.C. ; Reinink, Kees ; Schut, Johan W. ; Van Der Zeeuw, Eveline ; Vogelaar, Aat ; Freymark, Gerald ; Gutteling, Evert W. ; Keppel, Marina N. ; Van Drongelen, Paul ; Kieny, Matthieu ; Ellul, Philippe ; Touraev, Alisher ; Ma, Hong ; De Jong, Hans ; Wijnker, Erik. / Reverse breeding : A novel breeding approach based on engineered meiosis. In: Plant Biotechnology Journal. 2009 ; Vol. 7, No. 9. pp. 837-845.
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Dirks, R, Van Dun, K, De Snoo, CB, Van Den Berg, M, Lelivelt, CLC, Voermans, W, Woudenberg, L, De Wit, JPC, Reinink, K, Schut, JW, Van Der Zeeuw, E, Vogelaar, A, Freymark, G, Gutteling, EW, Keppel, MN, Van Drongelen, P, Kieny, M, Ellul, P, Touraev, A, Ma, H, De Jong, H & Wijnker, E 2009, 'Reverse breeding: A novel breeding approach based on engineered meiosis', Plant Biotechnology Journal, vol. 7, no. 9, pp. 837-845. https://doi.org/10.1111/j.1467-7652.2009.00450.x

Reverse breeding : A novel breeding approach based on engineered meiosis. / Dirks, Rob; Van Dun, Kees; De Snoo, C. Bastiaan; Van Den Berg, Mark; Lelivelt, Cilia L.C.; Voermans, William; Woudenberg, Leo; De Wit, Jack P.C.; Reinink, Kees; Schut, Johan W.; Van Der Zeeuw, Eveline; Vogelaar, Aat; Freymark, Gerald; Gutteling, Evert W.; Keppel, Marina N.; Van Drongelen, Paul; Kieny, Matthieu; Ellul, Philippe; Touraev, Alisher; Ma, Hong; De Jong, Hans; Wijnker, Erik.

In: Plant Biotechnology Journal, Vol. 7, No. 9, 01.12.2009, p. 837-845.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Reverse breeding

T2 - A novel breeding approach based on engineered meiosis

AU - Dirks, Rob

AU - Van Dun, Kees

AU - De Snoo, C. Bastiaan

AU - Van Den Berg, Mark

AU - Lelivelt, Cilia L.C.

AU - Voermans, William

AU - Woudenberg, Leo

AU - De Wit, Jack P.C.

AU - Reinink, Kees

AU - Schut, Johan W.

AU - Van Der Zeeuw, Eveline

AU - Vogelaar, Aat

AU - Freymark, Gerald

AU - Gutteling, Evert W.

AU - Keppel, Marina N.

AU - Van Drongelen, Paul

AU - Kieny, Matthieu

AU - Ellul, Philippe

AU - Touraev, Alisher

AU - Ma, Hong

AU - De Jong, Hans

AU - Wijnker, Erik

PY - 2009/12/1

Y1 - 2009/12/1

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Dirks R, Van Dun K, De Snoo CB, Van Den Berg M, Lelivelt CLC, Voermans W et al. Reverse breeding: A novel breeding approach based on engineered meiosis. Plant Biotechnology Journal. 2009 Dec 1;7(9):837-845. https://doi.org/10.1111/j.1467-7652.2009.00450.x