Reverse transcriptase- and RNA packaging signal-dependent incorporation of APOBEC3G into hepatitis B virus nucleocapsids

David H. Nguyen, Jianming Hu

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

APOBEC3G (A3G) is a cytidine deaminase that can inhibit a wide range of retroviruses, including the para-retrovirus hepatitis B virus (HBV). The antiviral function of A3G depends on its incorporation into assembling viral particles. However, it remains enigmatic how A3G is specifically packaged into a variety of unrelated viruses. By adopting a native agarose gel electrophoresis assay that can specifically measure the levels of A3G incorporation into HBV nucleocapsids, we found that A3G is specifically packaged into replication-competent HBV nucleocapsids in a fashion that is dependent on both the viral reverse transcriptase (RT) and viral RNA packaging signal, ε. In contrast, A3G is not incorporated into empty capsids formed in the absence of RT or ε. We demonstrated that the packaged A3G was protected from protease digestion by the nucleocapsids, thus confirming its interior localization. We also showed that A3G could bind RT specifically in an RNA-independent manner, which may be responsible for mediating the specific incorporation of A3G into replication-competent nucleocapsids. Finally, we provide evidence that the N-terminal domain of A3G is required for packaging into HBV nucleocapsids.

Original languageEnglish (US)
Pages (from-to)6852-6861
Number of pages10
JournalJournal of virology
Volume82
Issue number14
DOIs
StatePublished - Jul 1 2008

Fingerprint

Nucleocapsid
nucleocapsid
Hepatitis B virus
RNA-directed DNA polymerase
RNA-Directed DNA Polymerase
Product Packaging
RNA
Retroviridae
Cytidine Deaminase
Virus Assembly
capsid
Agar Gel Electrophoresis
Capsid
Viral RNA
virion
Virion
agarose
gel electrophoresis
packaging
Antiviral Agents

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

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title = "Reverse transcriptase- and RNA packaging signal-dependent incorporation of APOBEC3G into hepatitis B virus nucleocapsids",
abstract = "APOBEC3G (A3G) is a cytidine deaminase that can inhibit a wide range of retroviruses, including the para-retrovirus hepatitis B virus (HBV). The antiviral function of A3G depends on its incorporation into assembling viral particles. However, it remains enigmatic how A3G is specifically packaged into a variety of unrelated viruses. By adopting a native agarose gel electrophoresis assay that can specifically measure the levels of A3G incorporation into HBV nucleocapsids, we found that A3G is specifically packaged into replication-competent HBV nucleocapsids in a fashion that is dependent on both the viral reverse transcriptase (RT) and viral RNA packaging signal, ε. In contrast, A3G is not incorporated into empty capsids formed in the absence of RT or ε. We demonstrated that the packaged A3G was protected from protease digestion by the nucleocapsids, thus confirming its interior localization. We also showed that A3G could bind RT specifically in an RNA-independent manner, which may be responsible for mediating the specific incorporation of A3G into replication-competent nucleocapsids. Finally, we provide evidence that the N-terminal domain of A3G is required for packaging into HBV nucleocapsids.",
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Reverse transcriptase- and RNA packaging signal-dependent incorporation of APOBEC3G into hepatitis B virus nucleocapsids. / Nguyen, David H.; Hu, Jianming.

In: Journal of virology, Vol. 82, No. 14, 01.07.2008, p. 6852-6861.

Research output: Contribution to journalArticle

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