Reversed-phase liquid chromatographic isolation of lubimin and solavetivone from Hyoscyamus muticus "hairy" root cultures

G. Ramakrishna Reddy, Mark W. Signs, H. E. Flores, W. R. Curtis

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

A high-performance liquid chromatographic reversed-phase procedure was developed to estimate the concentration of sesquiterpene phytoalexins secreted into the media by Agrobacterium transformed root cultures of Hyoscyamus muticus. An isolation procedure based on solid-phase extraction (C18 Sep-pak) results in very rapid analysis and gives full recovery, compared to sequential chloroform extraction. A simple isocratic procedure permits quantification of sesquiterpene production by these root cultures in response to exposure to fungal elicitors. Calibration curves for lubimin and solavetivone content were obtained by fractionating sufficient material to provide a measurable weight. The identity of these compounds was confirmed by TLC, UV, GC-MS, and NMR. This experimental system is being used to examine the regulation of sesquiterpene biosynthesis, and to develop large-scale processing techniques for the production of secondary metabolises from plant roots grown in bioreactor systems.

Original languageEnglish (US)
Pages (from-to)440-444
Number of pages5
JournalJournal of Chromatography A
Volume657
Issue number2
DOIs
StatePublished - Dec 31 1993

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Reversed-phase liquid chromatographic isolation of lubimin and solavetivone from Hyoscyamus muticus "hairy" root cultures'. Together they form a unique fingerprint.

  • Cite this