TY - JOUR
T1 - Reversed-phase liquid chromatography of the twenty-two tetrachlorodibenzo-p-dioxin isomers on pyrenylethyl- and octadecylsilylated silica gel columns
AU - Barnhart, Elizabeth R.
AU - Patterson, Donald G.
AU - Tanaka, Nobuo
AU - Araki, Mikio
N1 - Funding Information:
This report was fully supported by funds from the Comprehensive Environmental Response, Compensation, and Liability Act trust fund. We gratefully acknowledge the support of Leslie Gelbaum (presently Manager of the NMR Facility, Center for Biotechnology, Georgia Institute of Technology) and Vikram Reddy for synthesis of the dioxin congeners and James Grainger for helpful discussions of IR data and structureeactivity relationships of TCDDs. We thank Wayman E. Turner for assistance with computer graphics. We are indebted to David Ashley for the NMR analyses and Louis Alexander for the GC-MS analysis. We especially thank Yuji Tachibana for the measurementso f the retention of the substituted benzenes on PYE and Cis.
PY - 1988
Y1 - 1988
N2 - To purify the tetrachlorobenzo-p-dioxins synthesized for chemical and biological reference standards, reversed-phase liquid chromatography on pyrenylethyl- and octadecylsilylated stationary phase (PYE and C18) columns was employed with 100% methanol. The pyrenylethyl phase satisfactorily separated isomers resulting from mixtures of reaction products which had not been adequately separated with conventional C18 or silica gel. The use of a single chromatographic mode, liquid chromatography, and a single mobile phase with columns of distinctly different properties, C18 and PYE, separates 20 of the 22 isomers from each other and from the 1246/1249 pair. (The observed properties of the two columns afford insight into steric and polarizability differences among isomers.
AB - To purify the tetrachlorobenzo-p-dioxins synthesized for chemical and biological reference standards, reversed-phase liquid chromatography on pyrenylethyl- and octadecylsilylated stationary phase (PYE and C18) columns was employed with 100% methanol. The pyrenylethyl phase satisfactorily separated isomers resulting from mixtures of reaction products which had not been adequately separated with conventional C18 or silica gel. The use of a single chromatographic mode, liquid chromatography, and a single mobile phase with columns of distinctly different properties, C18 and PYE, separates 20 of the 22 isomers from each other and from the 1246/1249 pair. (The observed properties of the two columns afford insight into steric and polarizability differences among isomers.
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U2 - 10.1016/S0021-9673(01)84516-7
DO - 10.1016/S0021-9673(01)84516-7
M3 - Article
C2 - 2851013
AN - SCOPUS:0024281648
VL - 445
SP - 145
EP - 154
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - C
ER -