TY - JOUR
T1 - Reversed-phase liquid chromatography of the twenty-two tetrachlorodibenzo-p-dioxin isomers on pyrenylethyl- and octadecylsilylated silica gel columns
AU - Barnhart, Elizabeth R.
AU - Patterson, Donald G.
AU - Tanaka, Nobuo
AU - Araki, Mikio
PY - 1988
Y1 - 1988
N2 - To purify the tetrachlorobenzo-p-dioxins synthesized for chemical and biological reference standards, reversed-phase liquid chromatography on pyrenylethyl- and octadecylsilylated stationary phase (PYE and C18) columns was employed with 100% methanol. The pyrenylethyl phase satisfactorily separated isomers resulting from mixtures of reaction products which had not been adequately separated with conventional C18 or silica gel. The use of a single chromatographic mode, liquid chromatography, and a single mobile phase with columns of distinctly different properties, C18 and PYE, separates 20 of the 22 isomers from each other and from the 1246/1249 pair. (The observed properties of the two columns afford insight into steric and polarizability differences among isomers.
AB - To purify the tetrachlorobenzo-p-dioxins synthesized for chemical and biological reference standards, reversed-phase liquid chromatography on pyrenylethyl- and octadecylsilylated stationary phase (PYE and C18) columns was employed with 100% methanol. The pyrenylethyl phase satisfactorily separated isomers resulting from mixtures of reaction products which had not been adequately separated with conventional C18 or silica gel. The use of a single chromatographic mode, liquid chromatography, and a single mobile phase with columns of distinctly different properties, C18 and PYE, separates 20 of the 22 isomers from each other and from the 1246/1249 pair. (The observed properties of the two columns afford insight into steric and polarizability differences among isomers.
UR - http://www.scopus.com/inward/record.url?scp=0024281648&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024281648&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(01)84516-7
DO - 10.1016/S0021-9673(01)84516-7
M3 - Article
C2 - 2851013
AN - SCOPUS:0024281648
VL - 445
SP - 145
EP - 154
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - C
ER -