Rhizobium meliloti and Rhizobium leguminosarum dctD gene products bind to tandem sites in an activation sequence located upstream to σ54-dependent dctA promoters

H. Ledebur, B. Gu, J. Sojda, B. Tracy Nixon

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Free-living rhizobia transport external C4-dicarboxylates to use as sole carbon sources, and uptake of these compounds is essential for nitrogen fixation by rhizobial bacteroides. In both Rhizobium leguminosarum and Rhizobium meliloti, the genes dctB and dctD are believed to form an ntrB/ntrC-like two-component system which regulates the synthesis of a C4-dicarboxylate transport protein encoded by dctA. Here we confirm the identity of σ54-dependent promoters previously hypothesized for the R. leguminosarum and R. meliloti dctA genes and demonstrate that repeated, partial dyad symmetry elements located about 75 base pairs upstream of each promoter are essential for fully regulated transcription. Furthermore, we show that both repeats bound dctD protein and that together they resulted in succinate-sensitive transcription when placed upstream of another σ54 consensus promoter, that of R. meliloti nifH.

Original languageEnglish (US)
Pages (from-to)3888-3897
Number of pages10
JournalJournal of Bacteriology
Volume172
Issue number7
DOIs
StatePublished - Jan 1 1990

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Rhizobium leguminosarum
Sinorhizobium meliloti
Dicarboxylic Acid Transporters
Genes
Nitrogen Fixation
Rhizobium
Bacteroides
Succinic Acid
Base Pairing
Carbon
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

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title = "Rhizobium meliloti and Rhizobium leguminosarum dctD gene products bind to tandem sites in an activation sequence located upstream to σ54-dependent dctA promoters",
abstract = "Free-living rhizobia transport external C4-dicarboxylates to use as sole carbon sources, and uptake of these compounds is essential for nitrogen fixation by rhizobial bacteroides. In both Rhizobium leguminosarum and Rhizobium meliloti, the genes dctB and dctD are believed to form an ntrB/ntrC-like two-component system which regulates the synthesis of a C4-dicarboxylate transport protein encoded by dctA. Here we confirm the identity of σ54-dependent promoters previously hypothesized for the R. leguminosarum and R. meliloti dctA genes and demonstrate that repeated, partial dyad symmetry elements located about 75 base pairs upstream of each promoter are essential for fully regulated transcription. Furthermore, we show that both repeats bound dctD protein and that together they resulted in succinate-sensitive transcription when placed upstream of another σ54 consensus promoter, that of R. meliloti nifH.",
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T1 - Rhizobium meliloti and Rhizobium leguminosarum dctD gene products bind to tandem sites in an activation sequence located upstream to σ54-dependent dctA promoters

AU - Ledebur, H.

AU - Gu, B.

AU - Sojda, J.

AU - Nixon, B. Tracy

PY - 1990/1/1

Y1 - 1990/1/1

N2 - Free-living rhizobia transport external C4-dicarboxylates to use as sole carbon sources, and uptake of these compounds is essential for nitrogen fixation by rhizobial bacteroides. In both Rhizobium leguminosarum and Rhizobium meliloti, the genes dctB and dctD are believed to form an ntrB/ntrC-like two-component system which regulates the synthesis of a C4-dicarboxylate transport protein encoded by dctA. Here we confirm the identity of σ54-dependent promoters previously hypothesized for the R. leguminosarum and R. meliloti dctA genes and demonstrate that repeated, partial dyad symmetry elements located about 75 base pairs upstream of each promoter are essential for fully regulated transcription. Furthermore, we show that both repeats bound dctD protein and that together they resulted in succinate-sensitive transcription when placed upstream of another σ54 consensus promoter, that of R. meliloti nifH.

AB - Free-living rhizobia transport external C4-dicarboxylates to use as sole carbon sources, and uptake of these compounds is essential for nitrogen fixation by rhizobial bacteroides. In both Rhizobium leguminosarum and Rhizobium meliloti, the genes dctB and dctD are believed to form an ntrB/ntrC-like two-component system which regulates the synthesis of a C4-dicarboxylate transport protein encoded by dctA. Here we confirm the identity of σ54-dependent promoters previously hypothesized for the R. leguminosarum and R. meliloti dctA genes and demonstrate that repeated, partial dyad symmetry elements located about 75 base pairs upstream of each promoter are essential for fully regulated transcription. Furthermore, we show that both repeats bound dctD protein and that together they resulted in succinate-sensitive transcription when placed upstream of another σ54 consensus promoter, that of R. meliloti nifH.

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