Abstract
Rhizobium meliloti DctD is believed to have three functional domains: an N‐terminal, two‐component receiver domain; and like other σ54‐dependent activators, C‐terminal and central domains for DNA binding and transcription activation. We have characterized a progressive series of M‐terminal deletions of R meliloti DctD. The N‐terminal domain was not needed for binding the dctA upstream activation sequence. Only 25% of the C‐terminal end of the receiver domain was needed to significantly inhibit the central domain, and proteins lacking up to 60% of the N‐terminal end of the receiver domain were‘inducible’in R. meliloti cells. We hypothesize that the W‐terminal two‐thirds of the DctD receiver domain augments and controls an adjacent subdomain for inhibiting the central domain.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 51-66 |
| Number of pages | 16 |
| Journal | Molecular Microbiology |
| Volume | 13 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1994 |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Molecular Biology
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Rhizobium meliloti DctD, a σ54‐dependent transcriptional activator, may be negatively controlled by a subdomain in the C‐terminal end of its two‐component receiver module. / Gu, Baohua; Lee, Joon H.; Hoover, Timothy R.; Scholl, Dean; Nixon, B. Tracy.
In: Molecular Microbiology, Vol. 13, No. 1, 01.01.1994, p. 51-66.Research output: Contribution to journal › Article
TY - JOUR
T1 - Rhizobium meliloti DctD, a σ54‐dependent transcriptional activator, may be negatively controlled by a subdomain in the C‐terminal end of its two‐component receiver module
AU - Gu, Baohua
AU - Lee, Joon H.
AU - Hoover, Timothy R.
AU - Scholl, Dean
AU - Nixon, B. Tracy
PY - 1994/1/1
Y1 - 1994/1/1
N2 - Rhizobium meliloti DctD is believed to have three functional domains: an N‐terminal, two‐component receiver domain; and like other σ54‐dependent activators, C‐terminal and central domains for DNA binding and transcription activation. We have characterized a progressive series of M‐terminal deletions of R meliloti DctD. The N‐terminal domain was not needed for binding the dctA upstream activation sequence. Only 25% of the C‐terminal end of the receiver domain was needed to significantly inhibit the central domain, and proteins lacking up to 60% of the N‐terminal end of the receiver domain were‘inducible’in R. meliloti cells. We hypothesize that the W‐terminal two‐thirds of the DctD receiver domain augments and controls an adjacent subdomain for inhibiting the central domain.
AB - Rhizobium meliloti DctD is believed to have three functional domains: an N‐terminal, two‐component receiver domain; and like other σ54‐dependent activators, C‐terminal and central domains for DNA binding and transcription activation. We have characterized a progressive series of M‐terminal deletions of R meliloti DctD. The N‐terminal domain was not needed for binding the dctA upstream activation sequence. Only 25% of the C‐terminal end of the receiver domain was needed to significantly inhibit the central domain, and proteins lacking up to 60% of the N‐terminal end of the receiver domain were‘inducible’in R. meliloti cells. We hypothesize that the W‐terminal two‐thirds of the DctD receiver domain augments and controls an adjacent subdomain for inhibiting the central domain.
UR - http://www.scopus.com/inward/record.url?scp=0028286432&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028286432&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2958.1994.tb00401.x
DO - 10.1111/j.1365-2958.1994.tb00401.x
M3 - Article
C2 - 7984094
AN - SCOPUS:0028286432
VL - 13
SP - 51
EP - 66
JO - Molecular Microbiology
JF - Molecular Microbiology
SN - 0950-382X
IS - 1
ER -