Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions

C. W. Ronson, B. T. Nixon, L. M. Albright, F. M. Ausubel

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

We report the identification and cloning of an ntrA-like (glnF rpoN) gene of Rhizobium meliloti and show that the R. meliloti ntrA product (NtrA) is required for C4-dicarboxylate transport as well as for nitrate assimilation and symbiotic nitrogen fixation. DNA sequence analysis showed that R. meliloti NtrA is 38% homologous with Klebsiella pneumoniae NtrA. Subcloning and complementation analysis suggested that the R. meliloti ntrA promoter lies within 125 base pairs of the initiation codon and may be constitutively expressed.

Original languageEnglish (US)
Pages (from-to)2424-2431
Number of pages8
JournalJournal of bacteriology
Volume169
Issue number6
DOIs
StatePublished - Jan 1 1987

Fingerprint

Sinorhizobium meliloti
Genes
Nitrogen Fixation
Initiator Codon
Klebsiella pneumoniae
DNA Sequence Analysis
Base Pairing
Nitrates
Organism Cloning

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Ronson, C. W. ; Nixon, B. T. ; Albright, L. M. ; Ausubel, F. M. / Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions. In: Journal of bacteriology. 1987 ; Vol. 169, No. 6. pp. 2424-2431.
@article{11dd7fcafea74973b835c8c5e185cf9f,
title = "Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions",
abstract = "We report the identification and cloning of an ntrA-like (glnF rpoN) gene of Rhizobium meliloti and show that the R. meliloti ntrA product (NtrA) is required for C4-dicarboxylate transport as well as for nitrate assimilation and symbiotic nitrogen fixation. DNA sequence analysis showed that R. meliloti NtrA is 38{\%} homologous with Klebsiella pneumoniae NtrA. Subcloning and complementation analysis suggested that the R. meliloti ntrA promoter lies within 125 base pairs of the initiation codon and may be constitutively expressed.",
author = "Ronson, {C. W.} and Nixon, {B. T.} and Albright, {L. M.} and Ausubel, {F. M.}",
year = "1987",
month = "1",
day = "1",
doi = "10.1128/jb.169.6.2424-2431.1987",
language = "English (US)",
volume = "169",
pages = "2424--2431",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "6",

}

Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions. / Ronson, C. W.; Nixon, B. T.; Albright, L. M.; Ausubel, F. M.

In: Journal of bacteriology, Vol. 169, No. 6, 01.01.1987, p. 2424-2431.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions

AU - Ronson, C. W.

AU - Nixon, B. T.

AU - Albright, L. M.

AU - Ausubel, F. M.

PY - 1987/1/1

Y1 - 1987/1/1

N2 - We report the identification and cloning of an ntrA-like (glnF rpoN) gene of Rhizobium meliloti and show that the R. meliloti ntrA product (NtrA) is required for C4-dicarboxylate transport as well as for nitrate assimilation and symbiotic nitrogen fixation. DNA sequence analysis showed that R. meliloti NtrA is 38% homologous with Klebsiella pneumoniae NtrA. Subcloning and complementation analysis suggested that the R. meliloti ntrA promoter lies within 125 base pairs of the initiation codon and may be constitutively expressed.

AB - We report the identification and cloning of an ntrA-like (glnF rpoN) gene of Rhizobium meliloti and show that the R. meliloti ntrA product (NtrA) is required for C4-dicarboxylate transport as well as for nitrate assimilation and symbiotic nitrogen fixation. DNA sequence analysis showed that R. meliloti NtrA is 38% homologous with Klebsiella pneumoniae NtrA. Subcloning and complementation analysis suggested that the R. meliloti ntrA promoter lies within 125 base pairs of the initiation codon and may be constitutively expressed.

UR - http://www.scopus.com/inward/record.url?scp=0023262822&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023262822&partnerID=8YFLogxK

U2 - 10.1128/jb.169.6.2424-2431.1987

DO - 10.1128/jb.169.6.2424-2431.1987

M3 - Article

C2 - 3034856

AN - SCOPUS:0023262822

VL - 169

SP - 2424

EP - 2431

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 6

ER -