Ribosomal DNA replication fork barrier and HOT1 recombination hot spot: Shared sequences but independent activities

Teresa R. Ward, Margaret L. Hoang, Reeta Prusty, Corine K. Lau, Ralph Keil, Walton L. Fangman, Bonita J. Brewer

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

In the ribosomal DNA of Saccharomyces cerevisiae, sequences in the nontranscribed spacer 3' of the 35S ribosomal RNA gene are important to the polar arrest of replication forks at a site called the replication fork barrier (RFB) and also to the cis-acting, mitotic hyperrecombination site called HOT1. We have found that the RFB and HOT1 activity share some but not all of their essential sequences. Many of the mutations that reduce HOT1 recombination also decrease or eliminate fork arrest at one of two closely spaced RFB sites, RFB1 and RFB2. A simple model for the juxtaposition of RFB and HOT1 sequences is that the breakage of strands in replication forks arrested at RFB stimulates recombination. Contrary to this model, we show here that HOT1-stimulated recombination does not require the arrest of forks at the RFB. Therefore, while HOT1 activity is independent of replication fork arrest, HOT1 and RFB require some common sequences, suggesting the existence of a common trans-acting factor(s).

Original languageEnglish (US)
Pages (from-to)4948-4957
Number of pages10
JournalMolecular and cellular biology
Volume20
Issue number13
DOIs
StatePublished - Jul 1 2000

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Ribosomal DNA
DNA Replication
Genetic Recombination
Trans-Activators
rRNA Genes
Saccharomyces cerevisiae
Mutation

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Ward, Teresa R. ; Hoang, Margaret L. ; Prusty, Reeta ; Lau, Corine K. ; Keil, Ralph ; Fangman, Walton L. ; Brewer, Bonita J. / Ribosomal DNA replication fork barrier and HOT1 recombination hot spot : Shared sequences but independent activities. In: Molecular and cellular biology. 2000 ; Vol. 20, No. 13. pp. 4948-4957.
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Ribosomal DNA replication fork barrier and HOT1 recombination hot spot : Shared sequences but independent activities. / Ward, Teresa R.; Hoang, Margaret L.; Prusty, Reeta; Lau, Corine K.; Keil, Ralph; Fangman, Walton L.; Brewer, Bonita J.

In: Molecular and cellular biology, Vol. 20, No. 13, 01.07.2000, p. 4948-4957.

Research output: Contribution to journalArticle

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