Ribosomal protein L10(L12) 4 autoregulates expression of the Bacillus subtilis rplJL operon by a transcription attenuation mechanism

Helen Yakhnin, Alexander V. Yakhnin, Paul Babitzke

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Ribosomal protein genes are of ten controlled by auto regulatory mechanisms in which a protein encoded in the operon can either bind to newly synthesized rRNA during rapid growth or to a similar target in its mRNA during poor growth conditions. The rplJL operon encodes the ribosomal L10(L12) 4 complex. In Escherichia coli L10(L12) 4 represses its translation by binding to the rplJL leader transcript. We identified three RNA structures in the Bacillus subtilis rplJL leader transcript that function as an antiantiterminator, antiterminator or intrinsic terminator. Expression studies with transcriptional and translational fusions indicated that L10(L12) 4 represses rplJL expression at the transcriptional level. RNA-binding studies demonstrated that L10(L12) 4 stabilizes the anti-antiterminator structure, while in vitro transcription results indicated that L10(L12) 4 promotestermination. Disruption of anti-antiterminator, antiterminator or terminator function by competitor oligonucleotides in vitro and by mutations in vivo demonstrated that each structure functions as predicted.Thus, rplJL expression is regulated by anautogenous transcription attenuation mechanism inwhich L10(L12) 4 binding to the anti-antiterminatorstructure promotes termination. We also found thattranslation of a leader peptide increases rplJL expression, presumably by inhibiting Rho-dependent termination. Thus, the rplJL operon of B. subtilis is regulated by transcription attenuation and antitermination mechanisms.

Original languageEnglish (US)
Pages (from-to)7032-7043
Number of pages12
JournalNucleic acids research
Volume43
Issue number14
DOIs
StatePublished - Jan 1 2015

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L 012
Operon
Bacillus subtilis
RNA
Ribosomal Proteins
Growth
Protein Sorting Signals
Oligonucleotides
ribosomal protein L10
ribosomal protein L7-L12
Escherichia coli
Messenger RNA
Mutation
Genes

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

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title = "Ribosomal protein L10(L12) 4 autoregulates expression of the Bacillus subtilis rplJL operon by a transcription attenuation mechanism",
abstract = "Ribosomal protein genes are of ten controlled by auto regulatory mechanisms in which a protein encoded in the operon can either bind to newly synthesized rRNA during rapid growth or to a similar target in its mRNA during poor growth conditions. The rplJL operon encodes the ribosomal L10(L12) 4 complex. In Escherichia coli L10(L12) 4 represses its translation by binding to the rplJL leader transcript. We identified three RNA structures in the Bacillus subtilis rplJL leader transcript that function as an antiantiterminator, antiterminator or intrinsic terminator. Expression studies with transcriptional and translational fusions indicated that L10(L12) 4 represses rplJL expression at the transcriptional level. RNA-binding studies demonstrated that L10(L12) 4 stabilizes the anti-antiterminator structure, while in vitro transcription results indicated that L10(L12) 4 promotestermination. Disruption of anti-antiterminator, antiterminator or terminator function by competitor oligonucleotides in vitro and by mutations in vivo demonstrated that each structure functions as predicted.Thus, rplJL expression is regulated by anautogenous transcription attenuation mechanism inwhich L10(L12) 4 binding to the anti-antiterminatorstructure promotes termination. We also found thattranslation of a leader peptide increases rplJL expression, presumably by inhibiting Rho-dependent termination. Thus, the rplJL operon of B. subtilis is regulated by transcription attenuation and antitermination mechanisms.",
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Ribosomal protein L10(L12) 4 autoregulates expression of the Bacillus subtilis rplJL operon by a transcription attenuation mechanism. / Yakhnin, Helen; Yakhnin, Alexander V.; Babitzke, Paul.

In: Nucleic acids research, Vol. 43, No. 14, 01.01.2015, p. 7032-7043.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Ribosomal protein L10(L12) 4 autoregulates expression of the Bacillus subtilis rplJL operon by a transcription attenuation mechanism

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AU - Yakhnin, Alexander V.

AU - Babitzke, Paul

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N2 - Ribosomal protein genes are of ten controlled by auto regulatory mechanisms in which a protein encoded in the operon can either bind to newly synthesized rRNA during rapid growth or to a similar target in its mRNA during poor growth conditions. The rplJL operon encodes the ribosomal L10(L12) 4 complex. In Escherichia coli L10(L12) 4 represses its translation by binding to the rplJL leader transcript. We identified three RNA structures in the Bacillus subtilis rplJL leader transcript that function as an antiantiterminator, antiterminator or intrinsic terminator. Expression studies with transcriptional and translational fusions indicated that L10(L12) 4 represses rplJL expression at the transcriptional level. RNA-binding studies demonstrated that L10(L12) 4 stabilizes the anti-antiterminator structure, while in vitro transcription results indicated that L10(L12) 4 promotestermination. Disruption of anti-antiterminator, antiterminator or terminator function by competitor oligonucleotides in vitro and by mutations in vivo demonstrated that each structure functions as predicted.Thus, rplJL expression is regulated by anautogenous transcription attenuation mechanism inwhich L10(L12) 4 binding to the anti-antiterminatorstructure promotes termination. We also found thattranslation of a leader peptide increases rplJL expression, presumably by inhibiting Rho-dependent termination. Thus, the rplJL operon of B. subtilis is regulated by transcription attenuation and antitermination mechanisms.

AB - Ribosomal protein genes are of ten controlled by auto regulatory mechanisms in which a protein encoded in the operon can either bind to newly synthesized rRNA during rapid growth or to a similar target in its mRNA during poor growth conditions. The rplJL operon encodes the ribosomal L10(L12) 4 complex. In Escherichia coli L10(L12) 4 represses its translation by binding to the rplJL leader transcript. We identified three RNA structures in the Bacillus subtilis rplJL leader transcript that function as an antiantiterminator, antiterminator or intrinsic terminator. Expression studies with transcriptional and translational fusions indicated that L10(L12) 4 represses rplJL expression at the transcriptional level. RNA-binding studies demonstrated that L10(L12) 4 stabilizes the anti-antiterminator structure, while in vitro transcription results indicated that L10(L12) 4 promotestermination. Disruption of anti-antiterminator, antiterminator or terminator function by competitor oligonucleotides in vitro and by mutations in vivo demonstrated that each structure functions as predicted.Thus, rplJL expression is regulated by anautogenous transcription attenuation mechanism inwhich L10(L12) 4 binding to the anti-antiterminatorstructure promotes termination. We also found thattranslation of a leader peptide increases rplJL expression, presumably by inhibiting Rho-dependent termination. Thus, the rplJL operon of B. subtilis is regulated by transcription attenuation and antitermination mechanisms.

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