RING Finger Protein 11 Targets TBK1/IKKi Kinases to Inhibit Antiviral Signaling

Soratree Charoenthongtrakul, Linlin Gao, Kislay Parvatiyar, David Lee, Edward Harhaj

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A key feature of the innate antiviral immune response is a rapid nonspecific response to virus infection largely mediated by the induction and extracellular secretion of type I interferons (IFNs) that restrict virus replication. Cytoplasmic sensors such as RIG-I recognize viral RNA and trigger antiviral signaling pathways that upregulate IFN transcription. However, it remains largely unknown how antiviral signaling is negatively regulated to maintain homeostasis after the elimination of virus. In this report, we have identified the RING domain-containing protein RING finger 11 (RNF11) as a novel negative regulator of innate antiviral signaling. Overexpression of RNF11 downregulated IFN-β expression and enhanced viral replication whereas siRNA-mediated knockdown of RNF11 suppressed viral replication. RNF11 interacted with the noncanonical IKK kinases TBK1/IKKi and attenuated their Lys63-linked polyubiquitination by blocking interactions with the E3 ligase TRAF3. The inhibitory function of RNF11 was dependent on the ubiquitin-binding adaptor molecule TAX1BP1 which was required for RNF11 to target TBK1/IKKi. Collectively, these results indicate that RNF11 functions together with TAX1BP1 to restrict antiviral signaling and IFN-β production.

Original languageEnglish (US)
Article numbere53717
JournalPloS one
Volume8
Issue number1
DOIs
StatePublished - Jan 15 2013

Fingerprint

interferons
Antiviral Agents
phosphotransferases (kinases)
Phosphotransferases
virus replication
Viruses
Interferons
IKappaB kinase
Proteins
proteins
TNF Receptor-Associated Factor 3
viruses
ubiquitin
small interfering RNA
Interferon Type I
ligases
Ubiquitin-Protein Ligases
Viral RNA
Virus Diseases
Transcription

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Charoenthongtrakul, Soratree ; Gao, Linlin ; Parvatiyar, Kislay ; Lee, David ; Harhaj, Edward. / RING Finger Protein 11 Targets TBK1/IKKi Kinases to Inhibit Antiviral Signaling. In: PloS one. 2013 ; Vol. 8, No. 1.
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RING Finger Protein 11 Targets TBK1/IKKi Kinases to Inhibit Antiviral Signaling. / Charoenthongtrakul, Soratree; Gao, Linlin; Parvatiyar, Kislay; Lee, David; Harhaj, Edward.

In: PloS one, Vol. 8, No. 1, e53717, 15.01.2013.

Research output: Contribution to journalArticle

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