RNA 3' processing regulates hist one mRNA levels in a mammalian cell cycle mutant. A processing factor becomes limiting in G1-arrested cells

Bernhard Luscher, Daniel Schümperli

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

Post-transcriptional regulation of histone gene expression in a mouse mastocytoma cell cycle mutant (21-Tb) depends largely on conserved DNA sequences that are essential for RNA 3' processing. We have analyzed whether this regulation occurs at the level of RNA 3' processing. We show, by RNase mapping, that nuclear H4 mRNA precursors, which are hardly detectable in total RNA from exponentially dividing cells, accumulate in Gl-arrested cells, i.e. when mature mRNAs are drastically reduced. Furthermore, we show that a heat-labile component of the processing apparatus, recently identified in HeLa cell nuclear extracts, is limiting in extracts from Gl-arrested 21-Tb cells. In contrast, this activity is in excess in extracts from exponentially dividing cells, whereas both extracts contain similar amounts of snRNPs of the Sm serotype. These fluctuations in the heatlabile activity may generally contribute to proliferation or cell cycle dependent histone gene regulation.

Original languageEnglish (US)
Pages (from-to)1721-1726
Number of pages6
JournalEMBO Journal
Volume6
Issue number6
DOIs
StatePublished - Jun 1987

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'RNA 3' processing regulates hist one mRNA levels in a mammalian cell cycle mutant. A processing factor becomes limiting in G1-arrested cells'. Together they form a unique fingerprint.

Cite this