RNA Fibers as Optimized Nanoscaffolds for siRNA Coordination and Reduced Immunological Recognition

Lauren Rackley, Jaimie Marie Stewart, Jacqueline Salotti, Andrey Krokhotin, Ankit Shah, Justin R. Halman, Ridhima Juneja, Jaclyn Smollett, Lauren Lee, Kyle Roark, Mathias Viard, Mubin Tarannum, Juan Vivero-Escoto, Peter F. Johnson, Marina A. Dobrovolskaia, Nikolay Dokholyan, Elisa Franco, Kirill A. Afonin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

RNA is a versatile biomaterial that can be used to engineer nanoassemblies for personalized treatment of various diseases. Despite promising advancements, the design of RNA nanoassemblies with minimal recognition by the immune system remains a major challenge. Here, an approach is reported to engineer RNA fibrous structures to operate as a customizable platform for efficient coordination of siRNAs and for maintaining low immunostimulation. Functional RNA fibers are studied in silico and their formation is confirmed by various experimental techniques and visualized by atomic force microscopy (AFM). It is demonstrated that the RNA fibers offer multiple advantages among which are: i) programmability and modular design that allow for simultaneous controlled delivery of multiple siRNAs and fluorophores, ii) reduced immunostimulation when compared to other programmable RNA nanoassemblies, and iii) simple production protocol for endotoxin-free fibers with the option of their cotranscriptional assembly. Furthermore, it is shown that functional RNA fibers can be efficiently delivered with various organic and inorganic carriers while retaining their structural integrity in cells. Specific gene silencing triggered by RNA fibers is assessed in human breast cancer and melanoma cell lines, with the confirmed ability of functional fibers to selectively target single nucleotide mutations.

Original languageEnglish (US)
Article number1805959
JournalAdvanced Functional Materials
Volume28
Issue number48
DOIs
StatePublished - Nov 28 2018

Fingerprint

RNA
Small Interfering RNA
fibers
Fibers
engineers
endotoxins
immune systems
nucleotides
mutations
retaining
cultured cells
Engineers
breast
genes
integrity
Fluorophores
Immune system
delivery
Biocompatible Materials
Structural integrity

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Rackley, L., Stewart, J. M., Salotti, J., Krokhotin, A., Shah, A., Halman, J. R., ... Afonin, K. A. (2018). RNA Fibers as Optimized Nanoscaffolds for siRNA Coordination and Reduced Immunological Recognition. Advanced Functional Materials, 28(48), [1805959]. https://doi.org/10.1002/adfm.201805959
Rackley, Lauren ; Stewart, Jaimie Marie ; Salotti, Jacqueline ; Krokhotin, Andrey ; Shah, Ankit ; Halman, Justin R. ; Juneja, Ridhima ; Smollett, Jaclyn ; Lee, Lauren ; Roark, Kyle ; Viard, Mathias ; Tarannum, Mubin ; Vivero-Escoto, Juan ; Johnson, Peter F. ; Dobrovolskaia, Marina A. ; Dokholyan, Nikolay ; Franco, Elisa ; Afonin, Kirill A. / RNA Fibers as Optimized Nanoscaffolds for siRNA Coordination and Reduced Immunological Recognition. In: Advanced Functional Materials. 2018 ; Vol. 28, No. 48.
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abstract = "RNA is a versatile biomaterial that can be used to engineer nanoassemblies for personalized treatment of various diseases. Despite promising advancements, the design of RNA nanoassemblies with minimal recognition by the immune system remains a major challenge. Here, an approach is reported to engineer RNA fibrous structures to operate as a customizable platform for efficient coordination of siRNAs and for maintaining low immunostimulation. Functional RNA fibers are studied in silico and their formation is confirmed by various experimental techniques and visualized by atomic force microscopy (AFM). It is demonstrated that the RNA fibers offer multiple advantages among which are: i) programmability and modular design that allow for simultaneous controlled delivery of multiple siRNAs and fluorophores, ii) reduced immunostimulation when compared to other programmable RNA nanoassemblies, and iii) simple production protocol for endotoxin-free fibers with the option of their cotranscriptional assembly. Furthermore, it is shown that functional RNA fibers can be efficiently delivered with various organic and inorganic carriers while retaining their structural integrity in cells. Specific gene silencing triggered by RNA fibers is assessed in human breast cancer and melanoma cell lines, with the confirmed ability of functional fibers to selectively target single nucleotide mutations.",
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Rackley, L, Stewart, JM, Salotti, J, Krokhotin, A, Shah, A, Halman, JR, Juneja, R, Smollett, J, Lee, L, Roark, K, Viard, M, Tarannum, M, Vivero-Escoto, J, Johnson, PF, Dobrovolskaia, MA, Dokholyan, N, Franco, E & Afonin, KA 2018, 'RNA Fibers as Optimized Nanoscaffolds for siRNA Coordination and Reduced Immunological Recognition', Advanced Functional Materials, vol. 28, no. 48, 1805959. https://doi.org/10.1002/adfm.201805959

RNA Fibers as Optimized Nanoscaffolds for siRNA Coordination and Reduced Immunological Recognition. / Rackley, Lauren; Stewart, Jaimie Marie; Salotti, Jacqueline; Krokhotin, Andrey; Shah, Ankit; Halman, Justin R.; Juneja, Ridhima; Smollett, Jaclyn; Lee, Lauren; Roark, Kyle; Viard, Mathias; Tarannum, Mubin; Vivero-Escoto, Juan; Johnson, Peter F.; Dobrovolskaia, Marina A.; Dokholyan, Nikolay; Franco, Elisa; Afonin, Kirill A.

In: Advanced Functional Materials, Vol. 28, No. 48, 1805959, 28.11.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - RNA Fibers as Optimized Nanoscaffolds for siRNA Coordination and Reduced Immunological Recognition

AU - Rackley, Lauren

AU - Stewart, Jaimie Marie

AU - Salotti, Jacqueline

AU - Krokhotin, Andrey

AU - Shah, Ankit

AU - Halman, Justin R.

AU - Juneja, Ridhima

AU - Smollett, Jaclyn

AU - Lee, Lauren

AU - Roark, Kyle

AU - Viard, Mathias

AU - Tarannum, Mubin

AU - Vivero-Escoto, Juan

AU - Johnson, Peter F.

AU - Dobrovolskaia, Marina A.

AU - Dokholyan, Nikolay

AU - Franco, Elisa

AU - Afonin, Kirill A.

PY - 2018/11/28

Y1 - 2018/11/28

N2 - RNA is a versatile biomaterial that can be used to engineer nanoassemblies for personalized treatment of various diseases. Despite promising advancements, the design of RNA nanoassemblies with minimal recognition by the immune system remains a major challenge. Here, an approach is reported to engineer RNA fibrous structures to operate as a customizable platform for efficient coordination of siRNAs and for maintaining low immunostimulation. Functional RNA fibers are studied in silico and their formation is confirmed by various experimental techniques and visualized by atomic force microscopy (AFM). It is demonstrated that the RNA fibers offer multiple advantages among which are: i) programmability and modular design that allow for simultaneous controlled delivery of multiple siRNAs and fluorophores, ii) reduced immunostimulation when compared to other programmable RNA nanoassemblies, and iii) simple production protocol for endotoxin-free fibers with the option of their cotranscriptional assembly. Furthermore, it is shown that functional RNA fibers can be efficiently delivered with various organic and inorganic carriers while retaining their structural integrity in cells. Specific gene silencing triggered by RNA fibers is assessed in human breast cancer and melanoma cell lines, with the confirmed ability of functional fibers to selectively target single nucleotide mutations.

AB - RNA is a versatile biomaterial that can be used to engineer nanoassemblies for personalized treatment of various diseases. Despite promising advancements, the design of RNA nanoassemblies with minimal recognition by the immune system remains a major challenge. Here, an approach is reported to engineer RNA fibrous structures to operate as a customizable platform for efficient coordination of siRNAs and for maintaining low immunostimulation. Functional RNA fibers are studied in silico and their formation is confirmed by various experimental techniques and visualized by atomic force microscopy (AFM). It is demonstrated that the RNA fibers offer multiple advantages among which are: i) programmability and modular design that allow for simultaneous controlled delivery of multiple siRNAs and fluorophores, ii) reduced immunostimulation when compared to other programmable RNA nanoassemblies, and iii) simple production protocol for endotoxin-free fibers with the option of their cotranscriptional assembly. Furthermore, it is shown that functional RNA fibers can be efficiently delivered with various organic and inorganic carriers while retaining their structural integrity in cells. Specific gene silencing triggered by RNA fibers is assessed in human breast cancer and melanoma cell lines, with the confirmed ability of functional fibers to selectively target single nucleotide mutations.

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