RNA isolation from recalcitrant plant tissue

David J. Schultz, Richard Craig, Diana L. Cox-Foster, Ralph O. Mumma, June I. Medford

Research output: Contribution to journalArticlepeer-review

122 Scopus citations

Abstract

The isolation of high-quality RNA from various tissues (leaves, pedicels, glandular trichomes) of garden geranium (Pelargonium xhortorum) using various published methods is difficult due to numerous oxidizing compounds. A new RNA extraction method was developed through the combination and modification of two separate procedures (Rochester et al., 1986; Manning 1991). In addition to geranium tissues, this method is successful when used with other recalcitrant tissues such as mature needles of white pine (Pinus strobus) and mature leaves of poinsettia (Euphorbia pulcherrima). RNA quality was judged by spectrophotometric readings, denaturing agarose gels, and successful reverse transcription.

Original languageEnglish (US)
Pages (from-to)310-316
Number of pages7
JournalPlant Molecular Biology Reporter
Volume12
Issue number4
DOIs
StatePublished - Dec 1 1994

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Plant Science

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