RNA Polymerase II Regulates Topoisomerase 1 Activity to Favor Efficient Transcription

Laura Baranello, Damian Wojtowicz, Kairong Cui, Ballachanda N. Devaiah, Hye Jung Chung, Ka Yim Chan-Salis, Rajarshi Guha, Kelli Wilson, Xiaohu Zhang, Hongliang Zhang, Jason Piotrowski, Craig J. Thomas, Dinah S. Singer, B. Franklin Pugh, Yves Pommier, Teresa M. Przytycka, Fedor Kouzine, Brian A. Lewis, Keji Zhao, David Levens

Research output: Contribution to journalArticlepeer-review

99 Scopus citations

Abstract

Summary We report a mechanism through which the transcription machinery directly controls topoisomerase 1 (TOP1) activity to adjust DNA topology throughout the transcription cycle. By comparing TOP1 occupancy using chromatin immunoprecipitation sequencing (ChIP-seq) versus TOP1 activity using topoisomerase 1 sequencing (TOP1-seq), a method reported here to map catalytically engaged TOP1, TOP1 bound at promoters was discovered to become fully active only after pause-release. This transition coupled the phosphorylation of the carboxyl-terminal-domain (CTD) of RNA polymerase II (RNAPII) with stimulation of TOP1 above its basal rate, enhancing its processivity. TOP1 stimulation is strongly dependent on the kinase activity of BRD4, a protein that phosphorylates Ser2-CTD and regulates RNAPII pause-release. Thus the coordinated action of BRD4 and TOP1 overcame the torsional stress opposing transcription as RNAPII commenced elongation but preserved negative supercoiling that assists promoter melting at start sites. This nexus between transcription and DNA topology promises to elicit new strategies to intercept pathological gene expression.

Original languageEnglish (US)
Pages (from-to)357-371
Number of pages15
JournalCell
Volume165
Issue number2
DOIs
StatePublished - Apr 7 2016

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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