RNA Secondary Structural Determinants of miRNA Precursor Processing in Arabidopsis

Liang Song, Michael J. Axtell, Nina V. Fedoroff

Research output: Contribution to journalArticle

114 Scopus citations

Abstract

MicroRNAs (miRNAs) are excised from hairpin structures within primary miRNAs (pri-miRNAs). Most animal pri-miRNAs are processed by two cleavages, the first at a loop-distal site ∼11 nucleotides (nt) from the end of the hairpin and the second ∼22 nt beyond the first [1-3]. To identify RNA structural determinants of miRNA processing in plants, we analyzed the functional consequences of changing the secondary structure of the lower (loop-distal), middle (miRNA:miRNA*), and upper (loop-proximal) stems of the hairpin in two different pri-miRNAs. Closing bulges immediately below the loop-distal cleavage sites increased the accumulation of accurately cleaved precursor miRNAs but decreased the abundance of the mature miRNAs. A pri-miRNA variant with an unpaired lower stem was not processed, and variants with a perfectly paired middle or upper stem were processed normally. Bioinformatic analysis of pri-miRNA structures, together with physical mapping of initial cleavage sites and in vitro processing of pri-miRNA, reveals that the first, loop-distal cleavage is often at a distance of ∼15 nt from an unpaired region. Hence, a common determinant of the rate and location of the initial pri-miRNA cleavage is an imperfectly base-paired duplex of ∼15 nt between the miRNA:miRNA* duplex and either a less structured region of the lower stem or its end.

Original languageEnglish (US)
Pages (from-to)37-41
Number of pages5
JournalCurrent Biology
Volume20
Issue number1
DOIs
StatePublished - Jan 12 2010

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Fingerprint Dive into the research topics of 'RNA Secondary Structural Determinants of miRNA Precursor Processing in Arabidopsis'. Together they form a unique fingerprint.

  • Cite this