Abstract
The homeodomain protein α2p plays a role both in transcriptional repression in the process of cell type determination and in donor selection during mating interconversion. We have explored the mechanism of α2p- directed donor selection by examining the effects on donor preference of mutants deficient in α2p-mediated transcriptional repression. As a transcriptional regulator, α2p interacts with Mcm1p, Tup1p, and Ssn6p to repress a-specific genes and with alp, Tnp1p, and Ssn6p to repress haploid- specific genes. We have found that mutant alleles of MATα2 that specifically diminish the interaction of α2p with Mcm1p or Tup1p behave as null alleles with regard to donor preference, while mutations of MATα2 that specifically diminish interaction of α2p with alp behave as wild-type MATα2 in this capacity. Tup1p plays an essential role in α2p-mediated transcriptional repression, while Ssn6p has only a modest effect in repression. In a similar vein, we find that TUP1, but not SSN6, is required for proper donor selection. These results suggest that, in addition to regulating a-specific gene expression to establish the mating type of the cell, α2p-Mcm1p-Tup1p complex may indirectly regulate donor preference through transcriptional control of an a-specific gene. Alternatively, this complex may play a direct role in establishing donor preference via its DNA binding and chromatin organization capacity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 751-759 |
| Number of pages | 9 |
| Journal | Molecular and cellular biology |
| Volume | 17 |
| Issue number | 2 |
| State | Published - Feb 1 1997 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology
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Role of 1/4 protein in donor locus selection during mating type interconversion. / Szeto, Lisa; Broach, James.
In: Molecular and cellular biology, Vol. 17, No. 2, 01.02.1997, p. 751-759.Research output: Contribution to journal › Article
TY - JOUR
T1 - Role of 1/4 protein in donor locus selection during mating type interconversion
AU - Szeto, Lisa
AU - Broach, James
PY - 1997/2/1
Y1 - 1997/2/1
N2 - The homeodomain protein α2p plays a role both in transcriptional repression in the process of cell type determination and in donor selection during mating interconversion. We have explored the mechanism of α2p- directed donor selection by examining the effects on donor preference of mutants deficient in α2p-mediated transcriptional repression. As a transcriptional regulator, α2p interacts with Mcm1p, Tup1p, and Ssn6p to repress a-specific genes and with alp, Tnp1p, and Ssn6p to repress haploid- specific genes. We have found that mutant alleles of MATα2 that specifically diminish the interaction of α2p with Mcm1p or Tup1p behave as null alleles with regard to donor preference, while mutations of MATα2 that specifically diminish interaction of α2p with alp behave as wild-type MATα2 in this capacity. Tup1p plays an essential role in α2p-mediated transcriptional repression, while Ssn6p has only a modest effect in repression. In a similar vein, we find that TUP1, but not SSN6, is required for proper donor selection. These results suggest that, in addition to regulating a-specific gene expression to establish the mating type of the cell, α2p-Mcm1p-Tup1p complex may indirectly regulate donor preference through transcriptional control of an a-specific gene. Alternatively, this complex may play a direct role in establishing donor preference via its DNA binding and chromatin organization capacity.
AB - The homeodomain protein α2p plays a role both in transcriptional repression in the process of cell type determination and in donor selection during mating interconversion. We have explored the mechanism of α2p- directed donor selection by examining the effects on donor preference of mutants deficient in α2p-mediated transcriptional repression. As a transcriptional regulator, α2p interacts with Mcm1p, Tup1p, and Ssn6p to repress a-specific genes and with alp, Tnp1p, and Ssn6p to repress haploid- specific genes. We have found that mutant alleles of MATα2 that specifically diminish the interaction of α2p with Mcm1p or Tup1p behave as null alleles with regard to donor preference, while mutations of MATα2 that specifically diminish interaction of α2p with alp behave as wild-type MATα2 in this capacity. Tup1p plays an essential role in α2p-mediated transcriptional repression, while Ssn6p has only a modest effect in repression. In a similar vein, we find that TUP1, but not SSN6, is required for proper donor selection. These results suggest that, in addition to regulating a-specific gene expression to establish the mating type of the cell, α2p-Mcm1p-Tup1p complex may indirectly regulate donor preference through transcriptional control of an a-specific gene. Alternatively, this complex may play a direct role in establishing donor preference via its DNA binding and chromatin organization capacity.
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M3 - Article
C2 - 9001229
AN - SCOPUS:0030614545
VL - 17
SP - 751
EP - 759
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 2
ER -