Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA

Kenneth C. Keiler; Patrick R.H. Waller; Robert T. Sauer

doi:10.1126/science.271.5251.990

Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA

Kenneth C. Keiler, Patrick R.H. Waller, Robert T. Sauer

Research output: Contribution to journal › Article › peer-review

907 Citations (SciVal)

Abstract

Variants of λ repressor and cytochrome b₅₆₂ translated from messenger RNAs without stop codons were modified by carboxyl terminal addition of an ssrA-encoded peptide tag and subsequently degraded by carboxyl terminal-specific proteases present in both the cytoplasm and periplasm of Escherichia coli. The tag appears to be added to the carboxyl terminus of the nascent polypeptide chain by cotranslational switching of the ribosome from the damaged messenger RNA to ssrA RNA.

Original language	English (US)
Pages (from-to)	990-993
Number of pages	4
Journal	Science
Volume	271
Issue number	5251
DOIs	https://doi.org/10.1126/science.271.5251.990
State	Published - Feb 16 1996

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abstract = "Variants of λ repressor and cytochrome b562 translated from messenger RNAs without stop codons were modified by carboxyl terminal addition of an ssrA-encoded peptide tag and subsequently degraded by carboxyl terminal-specific proteases present in both the cytoplasm and periplasm of Escherichia coli. The tag appears to be added to the carboxyl terminus of the nascent polypeptide chain by cotranslational switching of the ribosome from the damaged messenger RNA to ssrA RNA.",

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AU - Waller, Patrick R.H.

AU - Sauer, Robert T.

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ER -

Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA

Abstract

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