Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA

Kenneth C. Keiler, Patrick R.H. Waller, Robert T. Sauer

Research output: Contribution to journalArticlepeer-review

841 Scopus citations

Abstract

Variants of λ repressor and cytochrome b562 translated from messenger RNAs without stop codons were modified by carboxyl terminal addition of an ssrA-encoded peptide tag and subsequently degraded by carboxyl terminal-specific proteases present in both the cytoplasm and periplasm of Escherichia coli. The tag appears to be added to the carboxyl terminus of the nascent polypeptide chain by cotranslational switching of the ribosome from the damaged messenger RNA to ssrA RNA.

Original languageEnglish (US)
Pages (from-to)990-993
Number of pages4
JournalScience
Volume271
Issue number5251
DOIs
StatePublished - Feb 16 1996

All Science Journal Classification (ASJC) codes

  • General

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