Role of basolateral Na+-K+-Cl- cotransport in HCl secretion by amphibian gastric mucosa

David Soybel, S. R. Gullans, F. Maxwell, E. Delpire

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

In amphibians and mammals, luminal H+ secretion by the stomach requires Cl-. It is widely accepted that a basolateral Cl-/HCO3/- exchanger in the acid-secreting oxyntic cell restores the Cl- deficit resulting from apical HCl secretion. In this study, we used reverse transcriptase-polymerase chain reaction (RT-PCR) to generate a 1,200-bp fragment specific to a basolateral isoform of the Na+-K+-Cl- cotransporter in the gastric fundus of Necturus maculosus. By Northern analysis, we observed that gastric mucosa expresses greater than fivefold higher levels of mRNA encoding this cotransporter than any other tissue in the gastrointestinal tract. Feeding results in > 100% increases in mRNA levels in acid-secreting fundic mucosa but does not alter mRNA levels in the neighboring and non-acid-secreting antral mucosa or duodenum. Flux measurements using in vitro fundic mucosae indicate that acid secretion requires Na+ in the nutrient (i.e., serosal side) perfusate, is modulated by changes in nutrient K+ levels, and is inhibited by nutrient solutions containing 50 μM bumetanide, a recognized blocker of Na+-K+- Cl- cotransport. These findings suggest that this basolateral transporter plays a dominant and previously unsuspected role in secretion of HCl across the apical membrane.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume269
Issue number1 38-1
StatePublished - 1995

Fingerprint

gastric mucosa
Amphibians
Gastric Mucosa
amphibians
Mucous Membrane
secretion
mucosa
Messenger RNA
Acids
Necturus maculosus
Sodium-Potassium-Chloride Symporters
Chloride-Bicarbonate Antiporters
Gastric Parietal Cells
Gastric Fundus
Nutrients
Bumetanide
Food
acids
gastric fundus
Reverse Transcriptase Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Clinical Biochemistry
  • Physiology
  • Agricultural and Biological Sciences(all)

Cite this

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abstract = "In amphibians and mammals, luminal H+ secretion by the stomach requires Cl-. It is widely accepted that a basolateral Cl-/HCO3/- exchanger in the acid-secreting oxyntic cell restores the Cl- deficit resulting from apical HCl secretion. In this study, we used reverse transcriptase-polymerase chain reaction (RT-PCR) to generate a 1,200-bp fragment specific to a basolateral isoform of the Na+-K+-Cl- cotransporter in the gastric fundus of Necturus maculosus. By Northern analysis, we observed that gastric mucosa expresses greater than fivefold higher levels of mRNA encoding this cotransporter than any other tissue in the gastrointestinal tract. Feeding results in > 100{\%} increases in mRNA levels in acid-secreting fundic mucosa but does not alter mRNA levels in the neighboring and non-acid-secreting antral mucosa or duodenum. Flux measurements using in vitro fundic mucosae indicate that acid secretion requires Na+ in the nutrient (i.e., serosal side) perfusate, is modulated by changes in nutrient K+ levels, and is inhibited by nutrient solutions containing 50 μM bumetanide, a recognized blocker of Na+-K+- Cl- cotransport. These findings suggest that this basolateral transporter plays a dominant and previously unsuspected role in secretion of HCl across the apical membrane.",
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Role of basolateral Na+-K+-Cl- cotransport in HCl secretion by amphibian gastric mucosa. / Soybel, David; Gullans, S. R.; Maxwell, F.; Delpire, E.

In: American Journal of Physiology - Cell Physiology, Vol. 269, No. 1 38-1, 1995.

Research output: Contribution to journalArticle

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AU - Gullans, S. R.

AU - Maxwell, F.

AU - Delpire, E.

PY - 1995

Y1 - 1995

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