Role of Bordetella O antigen in respiratory tract infection

Valorie C. Burns, Elizabeth J. Pishko, Andrew Preston, Duncan J. Maskell, Eric T. Harvill

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Lipopolysaccharide (LPS), as the major surface molecule of gram-negative bacteria, interacts with the host in complex ways, both inducing and protecting against aspects of inflammatory and adaptive immunity. The membrane-distal repeated carbohydrate structure of LPS, the O antigen, can prevent antibody functions and may vary as a mechanism of immune evasion. Genes of the wbm locus are required for the assembly of O antigen on the animal pathogen Bordetella bronchiseptica and the human pathogen B. parapertussis. However, the important human pathogen B. pertussis lacks these genes and a number of in vitro and in vivo characteristics associated with O antigen in other organisms. To determine the specific functions of O antigen in these closely related Bordetella subspecies, we compared wbm deletion (Δwbm) mutants of B. bronchiseptica and B. parapertussis in a variety of assays relevant to natural respiratory tract infection. Complement was not activated or depleted by wild-type bordetellae expressing O antigen, but both Δwbm mutants activated complement and were highly sensitive to complement-mediated killing in vitro. Although the O-antigen structures appear to be substantially similar, the two mutants differed strikingly in their defects within the respiratory tract. The B. parapertussis Δwbm mutant was severely defective in colonization of the tracheas and lungs of mice, while the B. bronchiseptica Δwbm mutant showed almost no defect. While in vitro characteristics such as serum resistance may be attributable to O antigen directly, the role of O antigen during infection appears to be more complex, possibly involving factors differing among the closely related bordetellae or different interactions between each one and its host.

Original languageEnglish (US)
Pages (from-to)86-94
Number of pages9
JournalInfection and Immunity
Volume71
Issue number1
DOIs
StatePublished - Jan 1 2003

Fingerprint

Bordetella
O Antigens
Respiratory Tract Infections
Bordetella bronchiseptica
Lipopolysaccharides
Immune Evasion
Whooping Cough
Adaptive Immunity
Trachea
Gram-Negative Bacteria
Respiratory System
Genes
Carbohydrates
Lung

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Burns, V. C., Pishko, E. J., Preston, A., Maskell, D. J., & Harvill, E. T. (2003). Role of Bordetella O antigen in respiratory tract infection. Infection and Immunity, 71(1), 86-94. https://doi.org/10.1128/IAI.71.1.86-94.2003
Burns, Valorie C. ; Pishko, Elizabeth J. ; Preston, Andrew ; Maskell, Duncan J. ; Harvill, Eric T. / Role of Bordetella O antigen in respiratory tract infection. In: Infection and Immunity. 2003 ; Vol. 71, No. 1. pp. 86-94.
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Burns, VC, Pishko, EJ, Preston, A, Maskell, DJ & Harvill, ET 2003, 'Role of Bordetella O antigen in respiratory tract infection', Infection and Immunity, vol. 71, no. 1, pp. 86-94. https://doi.org/10.1128/IAI.71.1.86-94.2003

Role of Bordetella O antigen in respiratory tract infection. / Burns, Valorie C.; Pishko, Elizabeth J.; Preston, Andrew; Maskell, Duncan J.; Harvill, Eric T.

In: Infection and Immunity, Vol. 71, No. 1, 01.01.2003, p. 86-94.

Research output: Contribution to journalArticle

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Burns VC, Pishko EJ, Preston A, Maskell DJ, Harvill ET. Role of Bordetella O antigen in respiratory tract infection. Infection and Immunity. 2003 Jan 1;71(1):86-94. https://doi.org/10.1128/IAI.71.1.86-94.2003