Role of calcium and differentiation in enhanced sesquiterpene elicitation from calcium alginate-immobilized plant tissue

Wayne R. Curtis, Ping Wang, Arthur Humphrey

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Calcium alginate immobilization of Hyoscyamus muticus plant tissue culture is shown to enhance the rapid formation of sesquiterpenes in response to exposure to extracts of the fungal pathogen Rhizoctonia solani. The studies were carried out with suspension-cultured cells and roots at low tissue loadings to demonstrate that the enhancement is not due to either the degree of differentiation or the presence of metabolic gradients. The fungally elicited response of root tissue is shown to be much less sensitive to both the physical and chemical environment than free cell suspensions. By examining the effects of the immobilization components (Ca2+, alginate), and immobilization in barium alginate gels, it is concluded that the enhanced sesquiterpene production in immobilized tissue is mediated through calcium.

Original languageEnglish (US)
Pages (from-to)554-557
Number of pages4
JournalEnzyme and Microbial Technology
Volume17
Issue number6
DOIs
StatePublished - Jan 1 1995

Fingerprint

Sesquiterpenes
Alginate
Calcium
Immobilization
Tissue
Suspensions
Tissue culture
Hyoscyamus
Pathogens
Rhizoctonia
Barium
Gels
Cells
Cultured Cells
alginic acid

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

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abstract = "Calcium alginate immobilization of Hyoscyamus muticus plant tissue culture is shown to enhance the rapid formation of sesquiterpenes in response to exposure to extracts of the fungal pathogen Rhizoctonia solani. The studies were carried out with suspension-cultured cells and roots at low tissue loadings to demonstrate that the enhancement is not due to either the degree of differentiation or the presence of metabolic gradients. The fungally elicited response of root tissue is shown to be much less sensitive to both the physical and chemical environment than free cell suspensions. By examining the effects of the immobilization components (Ca2+, alginate), and immobilization in barium alginate gels, it is concluded that the enhanced sesquiterpene production in immobilized tissue is mediated through calcium.",
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Role of calcium and differentiation in enhanced sesquiterpene elicitation from calcium alginate-immobilized plant tissue. / Curtis, Wayne R.; Wang, Ping; Humphrey, Arthur.

In: Enzyme and Microbial Technology, Vol. 17, No. 6, 01.01.1995, p. 554-557.

Research output: Contribution to journalArticle

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