Role of glycocalyx in leukocyte-endothelial cell adhesion

A. W. Mulivor, Herbert Herling Lipowsky

Research output: Contribution to journalArticle

210 Citations (Scopus)

Abstract

The binding of fluorescently labeled microspheres (FLMs, 0.1-μm diameter) coated with antibody (1a29) to ICAM-1 was studied in postcapillary venules during topical application of the chemoattractant N-formylmethionyl-leucyl-phenylalanine (fMLP). FLM adhesion to endothelial cells (ECs) increased dramatically from 50 to 150 spheres per 100-μm length of venule after superfusion of the mesentery with fMLP and equaled or exceeded levels of leukocyte (WBC) adhesion. Removal of the EC glycocalyx by micropipette infusion of the venule with heparinase increased FLM-EC adhesion to levels attained with fMLP. Subsequent application of fMLP did not increase FLM adhesion further, suggesting that the FLMs saturated all ICAM-1 binding sites. Perfusion with heparinase after suffusion with fMLP significantly increased FLM-EC adhesion above levels attained with fMLP. However, WBC adhesion fell because of possible removal of selectins necessary to maintain WBC rolling at the wall. It is concluded that the glycocalyx serves as a barrier to adhesion and that its shedding during natural activation of ECs may be an essential part of the inflammatory response.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume283
Issue number4 52-4
StatePublished - Oct 1 2002

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N-Formylmethionine Leucyl-Phenylalanine
Glycocalyx
Cell Adhesion
Leukocytes
Endothelial Cells
Venules
Heparin Lyase
Intercellular Adhesion Molecule-1
Selectins
Mesentery
Chemotactic Factors
Microspheres
Perfusion
Binding Sites
Antibodies

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Mulivor, A. W. ; Lipowsky, Herbert Herling. / Role of glycocalyx in leukocyte-endothelial cell adhesion. In: American Journal of Physiology - Heart and Circulatory Physiology. 2002 ; Vol. 283, No. 4 52-4.
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Role of glycocalyx in leukocyte-endothelial cell adhesion. / Mulivor, A. W.; Lipowsky, Herbert Herling.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 283, No. 4 52-4, 01.10.2002.

Research output: Contribution to journalArticle

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AU - Lipowsky, Herbert Herling

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