Role of heme oxygenase in pulmonary response to antigen challenge in sensitized rats in vivo

Yuxia Jia, Kiyohisa Sekizawa, Shoji Okinaga, Ria Lie, Hidetada Sasaki

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Heme oxygenase (HO) protects against oxidant-mediated lung injury. However, it is uncertain whether changes in HO activity modulate antigen-induced airway inflammation. We studied the effects of pretreatment with either hemoglobin, a HO inducer, or tin protoporphyrin (SnPP)-9, a specific HO inhibitor, on increases in pulmonary insufflation pressure (PIP) and plasma extravasation induced by intravenously injected ovalbumin (OA) antigen in rats sensitized to OA in vivo with Evans blue dye as a marker. Pretreatment with hemoglobin (300 mg/kg) significantly increased (p < 0.01) and that with SnPP-9 (50 μmol/kg) significantly decreased (p < 0.01) HO activity of the lung, but they failed to alter OA antigen (300 μg/kg)-induced increases in PIP. In contrast, hemoglobin pretreatment significantly decreased (p < 0.01) and SnPP-9 pretreatment significantly increased (p < 0.05) the leakage of dye induced by OA antigen in the trachea, main bronchi, and segmental bronchi. OA antigen-induced increases in plasma extravasation were also inhibited by superoxide dismutase (12,000 U/kg). These findings suggest the oxygen radicals are involved in increases in plasma extravasation induced by antigen challenge and HO protects against antigen-induced airway inflammation.

Original languageEnglish (US)
Pages (from-to)141-145
Number of pages5
JournalInternational Archives of Allergy and Immunology
Volume120
Issue number2
DOIs
StatePublished - Oct 27 1999

Fingerprint

Heme Oxygenase (Decyclizing)
Ovalbumin
Antigens
Lung
Insufflation
Hemoglobins
Bronchi
Coloring Agents
Inflammation
Pressure
Evans Blue
Lung Injury
Trachea
Oxidants
Superoxide Dismutase
Reactive Oxygen Species

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Jia, Yuxia ; Sekizawa, Kiyohisa ; Okinaga, Shoji ; Lie, Ria ; Sasaki, Hidetada. / Role of heme oxygenase in pulmonary response to antigen challenge in sensitized rats in vivo. In: International Archives of Allergy and Immunology. 1999 ; Vol. 120, No. 2. pp. 141-145.
@article{d9efa18abb1443b6a53d3767ff7f8c2c,
title = "Role of heme oxygenase in pulmonary response to antigen challenge in sensitized rats in vivo",
abstract = "Heme oxygenase (HO) protects against oxidant-mediated lung injury. However, it is uncertain whether changes in HO activity modulate antigen-induced airway inflammation. We studied the effects of pretreatment with either hemoglobin, a HO inducer, or tin protoporphyrin (SnPP)-9, a specific HO inhibitor, on increases in pulmonary insufflation pressure (PIP) and plasma extravasation induced by intravenously injected ovalbumin (OA) antigen in rats sensitized to OA in vivo with Evans blue dye as a marker. Pretreatment with hemoglobin (300 mg/kg) significantly increased (p < 0.01) and that with SnPP-9 (50 μmol/kg) significantly decreased (p < 0.01) HO activity of the lung, but they failed to alter OA antigen (300 μg/kg)-induced increases in PIP. In contrast, hemoglobin pretreatment significantly decreased (p < 0.01) and SnPP-9 pretreatment significantly increased (p < 0.05) the leakage of dye induced by OA antigen in the trachea, main bronchi, and segmental bronchi. OA antigen-induced increases in plasma extravasation were also inhibited by superoxide dismutase (12,000 U/kg). These findings suggest the oxygen radicals are involved in increases in plasma extravasation induced by antigen challenge and HO protects against antigen-induced airway inflammation.",
author = "Yuxia Jia and Kiyohisa Sekizawa and Shoji Okinaga and Ria Lie and Hidetada Sasaki",
year = "1999",
month = "10",
day = "27",
doi = "10.1159/000024232",
language = "English (US)",
volume = "120",
pages = "141--145",
journal = "International Archives of Allergy and Immunology",
issn = "1018-2438",
publisher = "S. Karger AG",
number = "2",

}

Role of heme oxygenase in pulmonary response to antigen challenge in sensitized rats in vivo. / Jia, Yuxia; Sekizawa, Kiyohisa; Okinaga, Shoji; Lie, Ria; Sasaki, Hidetada.

In: International Archives of Allergy and Immunology, Vol. 120, No. 2, 27.10.1999, p. 141-145.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Role of heme oxygenase in pulmonary response to antigen challenge in sensitized rats in vivo

AU - Jia, Yuxia

AU - Sekizawa, Kiyohisa

AU - Okinaga, Shoji

AU - Lie, Ria

AU - Sasaki, Hidetada

PY - 1999/10/27

Y1 - 1999/10/27

N2 - Heme oxygenase (HO) protects against oxidant-mediated lung injury. However, it is uncertain whether changes in HO activity modulate antigen-induced airway inflammation. We studied the effects of pretreatment with either hemoglobin, a HO inducer, or tin protoporphyrin (SnPP)-9, a specific HO inhibitor, on increases in pulmonary insufflation pressure (PIP) and plasma extravasation induced by intravenously injected ovalbumin (OA) antigen in rats sensitized to OA in vivo with Evans blue dye as a marker. Pretreatment with hemoglobin (300 mg/kg) significantly increased (p < 0.01) and that with SnPP-9 (50 μmol/kg) significantly decreased (p < 0.01) HO activity of the lung, but they failed to alter OA antigen (300 μg/kg)-induced increases in PIP. In contrast, hemoglobin pretreatment significantly decreased (p < 0.01) and SnPP-9 pretreatment significantly increased (p < 0.05) the leakage of dye induced by OA antigen in the trachea, main bronchi, and segmental bronchi. OA antigen-induced increases in plasma extravasation were also inhibited by superoxide dismutase (12,000 U/kg). These findings suggest the oxygen radicals are involved in increases in plasma extravasation induced by antigen challenge and HO protects against antigen-induced airway inflammation.

AB - Heme oxygenase (HO) protects against oxidant-mediated lung injury. However, it is uncertain whether changes in HO activity modulate antigen-induced airway inflammation. We studied the effects of pretreatment with either hemoglobin, a HO inducer, or tin protoporphyrin (SnPP)-9, a specific HO inhibitor, on increases in pulmonary insufflation pressure (PIP) and plasma extravasation induced by intravenously injected ovalbumin (OA) antigen in rats sensitized to OA in vivo with Evans blue dye as a marker. Pretreatment with hemoglobin (300 mg/kg) significantly increased (p < 0.01) and that with SnPP-9 (50 μmol/kg) significantly decreased (p < 0.01) HO activity of the lung, but they failed to alter OA antigen (300 μg/kg)-induced increases in PIP. In contrast, hemoglobin pretreatment significantly decreased (p < 0.01) and SnPP-9 pretreatment significantly increased (p < 0.05) the leakage of dye induced by OA antigen in the trachea, main bronchi, and segmental bronchi. OA antigen-induced increases in plasma extravasation were also inhibited by superoxide dismutase (12,000 U/kg). These findings suggest the oxygen radicals are involved in increases in plasma extravasation induced by antigen challenge and HO protects against antigen-induced airway inflammation.

UR - http://www.scopus.com/inward/record.url?scp=0032883121&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032883121&partnerID=8YFLogxK

U2 - 10.1159/000024232

DO - 10.1159/000024232

M3 - Article

C2 - 10545768

AN - SCOPUS:0032883121

VL - 120

SP - 141

EP - 145

JO - International Archives of Allergy and Immunology

JF - International Archives of Allergy and Immunology

SN - 1018-2438

IS - 2

ER -