Role of km23-1 in RhoA/actin-based cell migration

Qunyan Jin, Nageswara R. Pulipati, Weidong Zhou, Cory M. Staub, Lance A. Liotta, Kathleen Mulder

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

km23-1 was originally identified as a TGFß receptor-interacting protein that plays an important role in TGFß signaling. Moreover, km23-1 is actually part of an ancient superfamily of NTPase-regulatory proteins, widely represented in archaea and bacteria. To further elucidate the function of km23-1, we identified novel protein interacting partners for km23-1 by using tandem affinity purification (TAP) and tandem mass spectrometry (MS). Here we show that km23-1 interacted with a class of proteins involved in actin-based cell motility and modulation of the actin cytoskeleton. We further showed that km23-1 modulates the formation of a highly organized stress fiber network. More significantly, we demonstrated that knockdown (KD) of km23-1 decreased RhoA activation in Mv1Lu epithelial cells. Finally, our results demonstrated for the first time that depletion of km23-1 inhibited cell migration of human colon carcinoma cells (HCCCs) in wound-healing assays. Overall, our findings demonstrate that km23-1 regulates RhoA and motility-associated actin modulating proteins, suggesting that km23-1 may represent a novel target for anti-metastatic therapy.

Original languageEnglish (US)
Pages (from-to)333-338
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume428
Issue number3
DOIs
StatePublished - Nov 23 2012

Fingerprint

Cell Movement
Actins
Nucleoside-Triphosphatase
Receptor-Interacting Protein Serine-Threonine Kinases
Stress Fibers
Proteins
Archaea
Tandem Mass Spectrometry
Actin Cytoskeleton
Wound Healing
Purification
Mass spectrometry
Assays
Bacteria
Colon
Epithelial Cells
Chemical activation
Cells
Modulation
Carcinoma

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Jin, Qunyan ; Pulipati, Nageswara R. ; Zhou, Weidong ; Staub, Cory M. ; Liotta, Lance A. ; Mulder, Kathleen. / Role of km23-1 in RhoA/actin-based cell migration. In: Biochemical and Biophysical Research Communications. 2012 ; Vol. 428, No. 3. pp. 333-338.
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Role of km23-1 in RhoA/actin-based cell migration. / Jin, Qunyan; Pulipati, Nageswara R.; Zhou, Weidong; Staub, Cory M.; Liotta, Lance A.; Mulder, Kathleen.

In: Biochemical and Biophysical Research Communications, Vol. 428, No. 3, 23.11.2012, p. 333-338.

Research output: Contribution to journalArticle

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AU - Zhou, Weidong

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AU - Mulder, Kathleen

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