Role of polyamines in the synthesis of prolactin-regulated growth factors by experimental breast cancer in culture

Andrea Manni, Betty Badger, Carol Wright, S. Rafeeq Ahmed, Steven J. Santner, Gordon Luk

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Abstract

The present experiments were designed to test whether polyamines play an essential role in the synthesis of growth factors induced by ovine prolactin (oPRL), using the N-nitrosomethylurea (NMU)-induced rat mammary tumor cultured in the soft agar clonogenic assay. Conditioned media (CM) obtained from tumors treated with oPRL and the polyamine biosynthesis inhibitor α-difluoromethylornithine (DFMO) (1 mM) no longer exerted the colony-stimulating effect which was observed with oPRL-CM. Such growth-promoting activity was restored with conditioned media obtained from tumors treated with oPRL, DFMO, and increasing concentrations of spermidine from 1 to 500 μM. The colony-stimulating effect of the CM employed could not be accounted for by the contaminating presence in the media of oPRL, DFMO, and polyamines. These results indicate that in our system polyamines play an important role in the synthesis of oPRL-regulated growth factors.

Original languageEnglish (US)
Pages (from-to)191-196
Number of pages6
JournalBreast Cancer Research and Treatment
Volume10
Issue number2
DOIs
StatePublished - Nov 1 1987

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All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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