Role of sonic hedgehog in branchiomotor neuron induction in zebrafish

Anand Chandrasekhar, James T. Warren, Kana Takahashi, Heike E. Schauerte, Fredericus J.M. Van Eeden, Pascal Haffter, John Y. Kuwada

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

The role of zebrafish hedgehog genes in branchiomotor neuron development was analyzed by examining mutations that affect the expression of the hedgehog genes and by overexpressing these genes in embryos. In cyclops mutants, reduction in sonic hedgehog (shh) expression, and elimination of tiggy-winkle hedgehog (twhh) expression, correlated with reductions in branchiomotor neuron populations. Furthermore, branchiomotor neurons were restored in cyclops mutants when shh or twhh was overexpressed. These results suggest that Shh and/or Twhh play an important role in the induction of branchiomotor neurons in vivo. In sonic-you (syu) mutants, where Shh activity was reduced or eliminated due to mutations in shh, branchiomotor neurons were reduced in number in a rhombomere-specific fashion, but never eliminated. Similarly, spinal motor neurons were reduced, but not eliminated, in syu mutants. These results demonstrate that Shh is not solely responsible for inducing branchiomotor and spinal motor neurons, and suggest that Shh and Twhh may function as partially redundant signals for motor neuron induction in zebrafish.

Original languageEnglish (US)
Pages (from-to)101-115
Number of pages15
JournalMechanisms of Development
Volume76
Issue number1-2
DOIs
StatePublished - Aug 1998

All Science Journal Classification (ASJC) codes

  • Embryology
  • Developmental Biology

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