Role of the Ek Molecule in the Generation of Suppressor T Cells in Response to LDHB

C. N. BAXEVANIS, N. ISHII, Z. A. NAGY, J. KLEIN

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35 Scopus citations

Abstract

The role of the Ek (EαEβk) molecule in the generation of suppressor T (Ts) cells specific for lactate dehydrogenase B (LDHB) was studied using different approaches. First, lymph node cells from LDHB‐primed B1O.A(2R) (AkEk) nonresponder mice were shown to suppress the LDHB‐specific and Ak‐restricted proliferative response of T cells from the congenic responder strain B10.A(4R), which does not express E molecules (AkEo). Similarly, lymph node cells from primed CBA (AkEk) mice suppressed the anti‐LDHB response of Lyt‐1+Lyt‐2 T celfs (depleted of Lyt‐2‐bearing TS cells) from the same mice. Second, in vitro priming of 2R (AkEk) T cells with LDHB‐pulsed 4R (AkEo) antigen‐presenting cells (APC) generated T‐cell proliferation but not suppression. Third, nonresponder 2R mice were turned into responders by injecting them with LDHB‐pulsed 4R APC or monoclonal Ia.m7 antibody that blocks the Ek molecule. The data demonstrate that expression of Ek molecules by the APC is necessary to generate LDHB‐specific Ts cells, which in turn prevent the proliferation of Lyt‐l+Lyt‐2 (probably helper) cells recognizing the same antigen in the context of the Ak molecule.

Original languageEnglish (US)
Pages (from-to)25-31
Number of pages7
JournalScandinavian Journal of Immunology
Volume16
Issue number1
DOIs
StatePublished - Jul 1982

All Science Journal Classification (ASJC) codes

  • Immunology

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