Roles of Drosophila Kruppel-Homolog 1 in neuronal morphogenesis

Lei Shi, Suewei Lin, Yelena Grinberg, Yannick Beck, Christina M. Grozinger, Gene E. Robinson, Tzumin Lee

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Abstract

The molecular mechanisms underlying remodeling of neural networks remain largely unknown. In Drosophila, widespread neural remodeling occurs during metamorphosis, and is regulated by ecdysone. Kruppel-homolog 1 (Kr-h1) is a zinc finger transcription factor known to play a role in orchestrating ecdysone-regulated transcriptional pathways and, furthermore, implicated in governing axon morphogenesis. Interestingly, in honey bee workers, neural expression of the Apis mellifera homolog of Kr-h1 is enhanced during their transition to foraging behavior when there is increased neurite outgrowth, branching, and synapse formation. Here, we assessed the role(s) of KR-H1 in Drosophila neuronal remodeling and morphology. We characterized the effect of Kr-h1 expression on neuronal morphology through Drosophila larval, pupal, and adult stages. Increased expression of Kr-h1 led to reduced branching in individual neurons and gross morphological changes in the mushroom bodies (MBs), while knocking down Kr-h1 did not produce any obvious changes in neural morphology. Drosophila Kr-h1 is normally expressed when MB neurons do not undergo active morphogenesis, suggesting that it may play a role in inhibiting morphogenesis. Further, loss of endogenous KR-H1 enhanced the neuronal morphogenesis that is otherwise delayed due to defective TGF-β signaling. However, loss of KR-H1 alone did not affect neuronal morphogenesis. In addition, Kr-h1 expression remains strongly linked to ecdysone-regulated pathways: Kr-h1 expression is regulated by usp, which dimerizes to the ecdysone receptor, and Kr-h1 expression is essential for proper patterning of the ecdysone receptor isoforms in the late larval central nervous system. Thus, although KR-H1 has a potential for modulating neuronal morphogenesis, it appears physiologically involved in coordinating general ecdysone signaling.

Original languageEnglish (US)
Pages (from-to)1614-1626
Number of pages13
JournalDevelopmental Neurobiology
Volume67
Issue number12
DOIs
StatePublished - Oct 1 2007

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All Science Journal Classification (ASJC) codes

  • Developmental Neuroscience
  • Cellular and Molecular Neuroscience

Cite this

Shi, L., Lin, S., Grinberg, Y., Beck, Y., Grozinger, C. M., Robinson, G. E., & Lee, T. (2007). Roles of Drosophila Kruppel-Homolog 1 in neuronal morphogenesis. Developmental Neurobiology, 67(12), 1614-1626. https://doi.org/10.1002/dneu.20537