Roles of three Fusarium graminearum membrane Ca2+ channels in the formation of Ca2+ signatures, growth, development, pathogenicity and mycotoxin production

Hye Seon Kim, Jung Eun Kim, Hokyoung Son, Daniel Frailey, Robert Cirino, Yin Won Lee, Randall Duncan, Kirk J. Czymmek, Seogchan Kang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Similar to animals and plants, external stimuli cause dynamic spatial and temporal changes of cytoplasmic Ca2+ in fungi. Such changes are referred as the Ca2+ signature and control cellular responses by modulating the activity or location of diverse Ca2+-binding proteins (CBPs) and also indirectly affecting proteins that interact with CBPs. To understand the mechanism underpinning Ca2+ signaling, therefore, characterization of how Ca2+ moves to and from the cytoplasm to create Ca2+ signatures under different conditions is fundamental. Three genes encoding plasma membrane Ca2+ channels in a Fusarium graminearum strain that expresses a fluorescent protein-based Ca2+ indicator in the cytoplasm were mutagenized to investigate their roles in the generation of Ca2+ signatures under different growth conditions and genetic backgrounds. The genes disrupted include CCH1 and MID1, which encode a high affinity Ca2+ uptake system, and FIG1, encoding a low affinity Ca2+ channel. Resulting mutants were also analyzed for growth, development, pathogenicity and mycotoxin production to determine how loss of each of the genes alters these traits. To investigate whether individual genes influence the function and expression of other genes, phenotypes and Ca2+ signatures of their double and triple mutants, as well as their expression patterns, were analyzed.

Original languageEnglish (US)
Pages (from-to)30-46
Number of pages17
JournalFungal Genetics and Biology
Volume111
DOIs
StatePublished - Feb 1 2018

Fingerprint

Mycotoxins
Fusarium
Ion Channels
Growth and Development
Virulence
Genes
Carrier Proteins
Cytoplasm
Proteins
Fungi
Cell Membrane
Phenotype
Gene Expression
Growth

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Genetics

Cite this

Kim, Hye Seon ; Kim, Jung Eun ; Son, Hokyoung ; Frailey, Daniel ; Cirino, Robert ; Lee, Yin Won ; Duncan, Randall ; Czymmek, Kirk J. ; Kang, Seogchan. / Roles of three Fusarium graminearum membrane Ca2+ channels in the formation of Ca2+ signatures, growth, development, pathogenicity and mycotoxin production. In: Fungal Genetics and Biology. 2018 ; Vol. 111. pp. 30-46.
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Roles of three Fusarium graminearum membrane Ca2+ channels in the formation of Ca2+ signatures, growth, development, pathogenicity and mycotoxin production. / Kim, Hye Seon; Kim, Jung Eun; Son, Hokyoung; Frailey, Daniel; Cirino, Robert; Lee, Yin Won; Duncan, Randall; Czymmek, Kirk J.; Kang, Seogchan.

In: Fungal Genetics and Biology, Vol. 111, 01.02.2018, p. 30-46.

Research output: Contribution to journalArticle

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