Roles of three Fusarium oxysporum calcium ion (Ca2+) channels in generating Ca2+ signatures and controlling growth

Hye Seon Kim, Jung Eun Kim, Daniel Frailey, Anja Nohe, Randall Duncan, Kirk J. Czymmek, Seogchan Kang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Spatial and temporal changes of cytoplasmic calcium ions ([Ca2+]c), caused by external stimuli, are known as the Ca2+ signature and presumably control cellular and developmental responses. Multiple types of ion channels, pumps, and transporters on plasma and organellar membranes modulate influx and efflux of Ca2+ to and from the extracellular environment and internal Ca2+ stores to form Ca2+ signatures. Expression of a fluorescent protein-based Ca2+ probe, Cameleon YC3.60, in Fusarium oxysporum enabled us to study how disruption of three Ca2+ channel genes, including FoCCH1, FoMID1 and FoYVC1, affects Ca2+ signature formation at polarized hyphal tips and whether specific changes in the Ca2+ signature caused by these mutations are related to growth-related phenotypes. Resulting mutants displayed altered amplitude, interval, and duration of Ca2+ pulses under various external Ca2+ concentrations as well as changes in sporulation and growth. Loss of FoMID1 and FoCCH1, genes encoding putative plasma membrane channel proteins, had a major impact on Ca2+ signatures and growth, while disruption of FoYVC1, which encodes a vacuolar channel, only subtly affected both traits. Results from our study provide new insights into the underpinning of Ca2+ signaling in fungi and its role in controlling growth and also raise several new questions.

Original languageEnglish (US)
Pages (from-to)145-157
Number of pages13
JournalFungal Genetics and Biology
Volume82
DOIs
StatePublished - Jan 1 2015

Fingerprint

Fusarium
Calcium Channels
Growth
Ion Channels
Ion Pumps
Cell Membrane
Genes
Blood Proteins
Membrane Proteins
Fungi
Ions
Calcium
Phenotype
Mutation
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Genetics

Cite this

Kim, Hye Seon ; Kim, Jung Eun ; Frailey, Daniel ; Nohe, Anja ; Duncan, Randall ; Czymmek, Kirk J. ; Kang, Seogchan. / Roles of three Fusarium oxysporum calcium ion (Ca2+) channels in generating Ca2+ signatures and controlling growth. In: Fungal Genetics and Biology. 2015 ; Vol. 82. pp. 145-157.
@article{e621da2ad4bb4c4e91b4ef3a592b8539,
title = "Roles of three Fusarium oxysporum calcium ion (Ca2+) channels in generating Ca2+ signatures and controlling growth",
abstract = "Spatial and temporal changes of cytoplasmic calcium ions ([Ca2+]c), caused by external stimuli, are known as the Ca2+ signature and presumably control cellular and developmental responses. Multiple types of ion channels, pumps, and transporters on plasma and organellar membranes modulate influx and efflux of Ca2+ to and from the extracellular environment and internal Ca2+ stores to form Ca2+ signatures. Expression of a fluorescent protein-based Ca2+ probe, Cameleon YC3.60, in Fusarium oxysporum enabled us to study how disruption of three Ca2+ channel genes, including FoCCH1, FoMID1 and FoYVC1, affects Ca2+ signature formation at polarized hyphal tips and whether specific changes in the Ca2+ signature caused by these mutations are related to growth-related phenotypes. Resulting mutants displayed altered amplitude, interval, and duration of Ca2+ pulses under various external Ca2+ concentrations as well as changes in sporulation and growth. Loss of FoMID1 and FoCCH1, genes encoding putative plasma membrane channel proteins, had a major impact on Ca2+ signatures and growth, while disruption of FoYVC1, which encodes a vacuolar channel, only subtly affected both traits. Results from our study provide new insights into the underpinning of Ca2+ signaling in fungi and its role in controlling growth and also raise several new questions.",
author = "Kim, {Hye Seon} and Kim, {Jung Eun} and Daniel Frailey and Anja Nohe and Randall Duncan and Czymmek, {Kirk J.} and Seogchan Kang",
year = "2015",
month = "1",
day = "1",
doi = "10.1016/j.fgb.2015.07.003",
language = "English (US)",
volume = "82",
pages = "145--157",
journal = "Fungal Genetics and Biology",
issn = "1087-1845",
publisher = "Academic Press Inc.",

}

Roles of three Fusarium oxysporum calcium ion (Ca2+) channels in generating Ca2+ signatures and controlling growth. / Kim, Hye Seon; Kim, Jung Eun; Frailey, Daniel; Nohe, Anja; Duncan, Randall; Czymmek, Kirk J.; Kang, Seogchan.

In: Fungal Genetics and Biology, Vol. 82, 01.01.2015, p. 145-157.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Roles of three Fusarium oxysporum calcium ion (Ca2+) channels in generating Ca2+ signatures and controlling growth

AU - Kim, Hye Seon

AU - Kim, Jung Eun

AU - Frailey, Daniel

AU - Nohe, Anja

AU - Duncan, Randall

AU - Czymmek, Kirk J.

AU - Kang, Seogchan

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Spatial and temporal changes of cytoplasmic calcium ions ([Ca2+]c), caused by external stimuli, are known as the Ca2+ signature and presumably control cellular and developmental responses. Multiple types of ion channels, pumps, and transporters on plasma and organellar membranes modulate influx and efflux of Ca2+ to and from the extracellular environment and internal Ca2+ stores to form Ca2+ signatures. Expression of a fluorescent protein-based Ca2+ probe, Cameleon YC3.60, in Fusarium oxysporum enabled us to study how disruption of three Ca2+ channel genes, including FoCCH1, FoMID1 and FoYVC1, affects Ca2+ signature formation at polarized hyphal tips and whether specific changes in the Ca2+ signature caused by these mutations are related to growth-related phenotypes. Resulting mutants displayed altered amplitude, interval, and duration of Ca2+ pulses under various external Ca2+ concentrations as well as changes in sporulation and growth. Loss of FoMID1 and FoCCH1, genes encoding putative plasma membrane channel proteins, had a major impact on Ca2+ signatures and growth, while disruption of FoYVC1, which encodes a vacuolar channel, only subtly affected both traits. Results from our study provide new insights into the underpinning of Ca2+ signaling in fungi and its role in controlling growth and also raise several new questions.

AB - Spatial and temporal changes of cytoplasmic calcium ions ([Ca2+]c), caused by external stimuli, are known as the Ca2+ signature and presumably control cellular and developmental responses. Multiple types of ion channels, pumps, and transporters on plasma and organellar membranes modulate influx and efflux of Ca2+ to and from the extracellular environment and internal Ca2+ stores to form Ca2+ signatures. Expression of a fluorescent protein-based Ca2+ probe, Cameleon YC3.60, in Fusarium oxysporum enabled us to study how disruption of three Ca2+ channel genes, including FoCCH1, FoMID1 and FoYVC1, affects Ca2+ signature formation at polarized hyphal tips and whether specific changes in the Ca2+ signature caused by these mutations are related to growth-related phenotypes. Resulting mutants displayed altered amplitude, interval, and duration of Ca2+ pulses under various external Ca2+ concentrations as well as changes in sporulation and growth. Loss of FoMID1 and FoCCH1, genes encoding putative plasma membrane channel proteins, had a major impact on Ca2+ signatures and growth, while disruption of FoYVC1, which encodes a vacuolar channel, only subtly affected both traits. Results from our study provide new insights into the underpinning of Ca2+ signaling in fungi and its role in controlling growth and also raise several new questions.

UR - http://www.scopus.com/inward/record.url?scp=84938088593&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938088593&partnerID=8YFLogxK

U2 - 10.1016/j.fgb.2015.07.003

DO - 10.1016/j.fgb.2015.07.003

M3 - Article

C2 - 26162966

AN - SCOPUS:84938088593

VL - 82

SP - 145

EP - 157

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

SN - 1087-1845

ER -