Roots of hydroponically grown Taxus plants as a source of taxol and related taxanes

Enaksha R.M. Wickremesinhe, Richard N. Arteca

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Taxus x media Rehd. cultivars Brownii (TMB), Densiformis (TMD), Fieldii (TMF), Hicksii (TMH) and T. cuspidata Sieb. and Zucc. varities 'improved' (TC-Imp) and 'special' (TC-Sp) were grown hydroponically in Johnson's nutrient solution (JS) for 22 weeks, at which time they were harvested for analysis. One-tenth strength JS produced the highest biomass for all plants. Plants grown in either one-tenth or one-twentieth strength JS had significantly greater root growth, compared with shoot growth. The highest root to shoot ratio (1.9) was achieved by TC-Sp, followed by TC-Imp (1.8) and TMB (1.8). The concentrations of taxol and 10-deacetyltaxol in the roots of all plants were comparable with or greater than the amounts found in the 'above-ground' parts. TMB (212 ± 43 mg/kg), TMH (381 ± 25 mg/kg), and TC-Sp (317 ± 32 mg/kg) roots contained greater than 1.5-fold amounts of taxol, compared with their needles. However, there were no major differences between the needles and the roots with respect to both cephalomannine and 10-deacetyltaxol levels. The highest taxol concentration was observed in the root bark of TMH (619 ± 50 mg/kg taxol). Cumulative amounts of taxol, cephalomannine and 10-deacetyltaxol found in the root bark of TMH and TC-Imp totaled 1158 and 1159 mg/kg dry matter, respectively. The presence of taxol, cephalomanine and 10-deacetyltaxol was detected and quantified by high performance liquid chromatography, and the structure confirmed by fast atom bombardment mass spectrometry.

Original languageEnglish (US)
Pages (from-to)125-135
Number of pages11
JournalPlant Science
Volume101
Issue number2
DOIs
StatePublished - Jan 1 1994

Fingerprint

Taxus
taxanes
Taxoids
paclitaxel
Paclitaxel
cephalomannine
nutrient solutions
Needles
bark
Taxus media
Taxus cuspidata
Fast Atom Bombardment Mass Spectrometry
Plant Roots
root shoot ratio
Growth
Biomass
aerial parts
root growth
high performance liquid chromatography
High Pressure Liquid Chromatography

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Wickremesinhe, Enaksha R.M. ; Arteca, Richard N. / Roots of hydroponically grown Taxus plants as a source of taxol and related taxanes. In: Plant Science. 1994 ; Vol. 101, No. 2. pp. 125-135.
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Roots of hydroponically grown Taxus plants as a source of taxol and related taxanes. / Wickremesinhe, Enaksha R.M.; Arteca, Richard N.

In: Plant Science, Vol. 101, No. 2, 01.01.1994, p. 125-135.

Research output: Contribution to journalArticle

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AB - Taxus x media Rehd. cultivars Brownii (TMB), Densiformis (TMD), Fieldii (TMF), Hicksii (TMH) and T. cuspidata Sieb. and Zucc. varities 'improved' (TC-Imp) and 'special' (TC-Sp) were grown hydroponically in Johnson's nutrient solution (JS) for 22 weeks, at which time they were harvested for analysis. One-tenth strength JS produced the highest biomass for all plants. Plants grown in either one-tenth or one-twentieth strength JS had significantly greater root growth, compared with shoot growth. The highest root to shoot ratio (1.9) was achieved by TC-Sp, followed by TC-Imp (1.8) and TMB (1.8). The concentrations of taxol and 10-deacetyltaxol in the roots of all plants were comparable with or greater than the amounts found in the 'above-ground' parts. TMB (212 ± 43 mg/kg), TMH (381 ± 25 mg/kg), and TC-Sp (317 ± 32 mg/kg) roots contained greater than 1.5-fold amounts of taxol, compared with their needles. However, there were no major differences between the needles and the roots with respect to both cephalomannine and 10-deacetyltaxol levels. The highest taxol concentration was observed in the root bark of TMH (619 ± 50 mg/kg taxol). Cumulative amounts of taxol, cephalomannine and 10-deacetyltaxol found in the root bark of TMH and TC-Imp totaled 1158 and 1159 mg/kg dry matter, respectively. The presence of taxol, cephalomanine and 10-deacetyltaxol was detected and quantified by high performance liquid chromatography, and the structure confirmed by fast atom bombardment mass spectrometry.

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