Rootstock-regulated gene expression profiling in apple trees reveals genes whose expression levels are associated with fire blight resistance

P. J. Jensen, Timothy W. McNellis, Naomi S. Altman, Siela Maximova, Robert Michael Crassweller, N. Halbrendt, J. W. Travis, C. A. Praul, I. Makalowska

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Desirable apple varieties are clonally propagated by grafting vegetative scions onto rootstocks. Rootstocks influence many phenotypic traits of the scion, including disease resistance. Fire blight has become increasingly more of a problem with the shift to high-density orchard planting, as the strongly dwarfing rootstocks used are generally more susceptible to the disease. The susceptibilities of the major rootstocks are relatively well established. However the influence of the rootstock on the susceptibility of the scion is less well characterized. In the spring of 2007 we performed fire blight inoculations on 3 year old trees of 'Gala' grafted on seven different rootstocks and measured the growth of the resultant cankers. 'Gala' grafted on G.30 showed the lowest rate of canker growth while 'Gala' on M.27 showed the highest rate of canker growth. Using an apple DNA microarray, we examined the gene expression patterns in greenhouse grown 'Gala' scions grafted to the same series of seven different rootstocks that we examined for the susceptibilities to fire blight. Over 100 genes were identified whose expression levels correlated with the degree of fire blight susceptibility of the scion/rootstock combinations. We will use Quantitative PCR to test these correlations in an orchard-grown population of different scion/rootstocks combinations that have been rated for fire blight susceptibility. This study demonstrates the utility of using rootstock-regulated gene expression profiling for the identification of genes associated with agriculturally important traits. These genes may be useful for apple breeding programs.

Original languageEnglish (US)
Title of host publicationIX International Symposium on Integrating Canopy, Rootstock and Environmental Physiology in Orchard Systems
PublisherInternational Society for Horticultural Science
Pages87-93
Number of pages7
ISBN (Print)9789066052970
DOIs
StatePublished - Aug 1 2011

Publication series

NameActa Horticulturae
Volume903
ISSN (Print)0567-7572

Fingerprint

Erwinia amylovora
rootstocks
apples
gene expression
scions
rootstock-scion relationships
orchards
cankers (plants)
genes
dwarfing
biological resistance
grafting (plants)
disease resistance
quantitative polymerase chain reaction
planting
greenhouses
breeding

All Science Journal Classification (ASJC) codes

  • Horticulture

Cite this

Jensen, P. J., McNellis, T. W., Altman, N. S., Maximova, S., Crassweller, R. M., Halbrendt, N., ... Makalowska, I. (2011). Rootstock-regulated gene expression profiling in apple trees reveals genes whose expression levels are associated with fire blight resistance. In IX International Symposium on Integrating Canopy, Rootstock and Environmental Physiology in Orchard Systems (pp. 87-93). (Acta Horticulturae; Vol. 903). International Society for Horticultural Science. https://doi.org/10.17660/ActaHortic.2011.903.7
Jensen, P. J. ; McNellis, Timothy W. ; Altman, Naomi S. ; Maximova, Siela ; Crassweller, Robert Michael ; Halbrendt, N. ; Travis, J. W. ; Praul, C. A. ; Makalowska, I. / Rootstock-regulated gene expression profiling in apple trees reveals genes whose expression levels are associated with fire blight resistance. IX International Symposium on Integrating Canopy, Rootstock and Environmental Physiology in Orchard Systems. International Society for Horticultural Science, 2011. pp. 87-93 (Acta Horticulturae).
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abstract = "Desirable apple varieties are clonally propagated by grafting vegetative scions onto rootstocks. Rootstocks influence many phenotypic traits of the scion, including disease resistance. Fire blight has become increasingly more of a problem with the shift to high-density orchard planting, as the strongly dwarfing rootstocks used are generally more susceptible to the disease. The susceptibilities of the major rootstocks are relatively well established. However the influence of the rootstock on the susceptibility of the scion is less well characterized. In the spring of 2007 we performed fire blight inoculations on 3 year old trees of 'Gala' grafted on seven different rootstocks and measured the growth of the resultant cankers. 'Gala' grafted on G.30 showed the lowest rate of canker growth while 'Gala' on M.27 showed the highest rate of canker growth. Using an apple DNA microarray, we examined the gene expression patterns in greenhouse grown 'Gala' scions grafted to the same series of seven different rootstocks that we examined for the susceptibilities to fire blight. Over 100 genes were identified whose expression levels correlated with the degree of fire blight susceptibility of the scion/rootstock combinations. We will use Quantitative PCR to test these correlations in an orchard-grown population of different scion/rootstocks combinations that have been rated for fire blight susceptibility. This study demonstrates the utility of using rootstock-regulated gene expression profiling for the identification of genes associated with agriculturally important traits. These genes may be useful for apple breeding programs.",
author = "Jensen, {P. J.} and McNellis, {Timothy W.} and Altman, {Naomi S.} and Siela Maximova and Crassweller, {Robert Michael} and N. Halbrendt and Travis, {J. W.} and Praul, {C. A.} and I. Makalowska",
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Jensen, PJ, McNellis, TW, Altman, NS, Maximova, S, Crassweller, RM, Halbrendt, N, Travis, JW, Praul, CA & Makalowska, I 2011, Rootstock-regulated gene expression profiling in apple trees reveals genes whose expression levels are associated with fire blight resistance. in IX International Symposium on Integrating Canopy, Rootstock and Environmental Physiology in Orchard Systems. Acta Horticulturae, vol. 903, International Society for Horticultural Science, pp. 87-93. https://doi.org/10.17660/ActaHortic.2011.903.7

Rootstock-regulated gene expression profiling in apple trees reveals genes whose expression levels are associated with fire blight resistance. / Jensen, P. J.; McNellis, Timothy W.; Altman, Naomi S.; Maximova, Siela; Crassweller, Robert Michael; Halbrendt, N.; Travis, J. W.; Praul, C. A.; Makalowska, I.

IX International Symposium on Integrating Canopy, Rootstock and Environmental Physiology in Orchard Systems. International Society for Horticultural Science, 2011. p. 87-93 (Acta Horticulturae; Vol. 903).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Jensen, P. J.

AU - McNellis, Timothy W.

AU - Altman, Naomi S.

AU - Maximova, Siela

AU - Crassweller, Robert Michael

AU - Halbrendt, N.

AU - Travis, J. W.

AU - Praul, C. A.

AU - Makalowska, I.

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N2 - Desirable apple varieties are clonally propagated by grafting vegetative scions onto rootstocks. Rootstocks influence many phenotypic traits of the scion, including disease resistance. Fire blight has become increasingly more of a problem with the shift to high-density orchard planting, as the strongly dwarfing rootstocks used are generally more susceptible to the disease. The susceptibilities of the major rootstocks are relatively well established. However the influence of the rootstock on the susceptibility of the scion is less well characterized. In the spring of 2007 we performed fire blight inoculations on 3 year old trees of 'Gala' grafted on seven different rootstocks and measured the growth of the resultant cankers. 'Gala' grafted on G.30 showed the lowest rate of canker growth while 'Gala' on M.27 showed the highest rate of canker growth. Using an apple DNA microarray, we examined the gene expression patterns in greenhouse grown 'Gala' scions grafted to the same series of seven different rootstocks that we examined for the susceptibilities to fire blight. Over 100 genes were identified whose expression levels correlated with the degree of fire blight susceptibility of the scion/rootstock combinations. We will use Quantitative PCR to test these correlations in an orchard-grown population of different scion/rootstocks combinations that have been rated for fire blight susceptibility. This study demonstrates the utility of using rootstock-regulated gene expression profiling for the identification of genes associated with agriculturally important traits. These genes may be useful for apple breeding programs.

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Jensen PJ, McNellis TW, Altman NS, Maximova S, Crassweller RM, Halbrendt N et al. Rootstock-regulated gene expression profiling in apple trees reveals genes whose expression levels are associated with fire blight resistance. In IX International Symposium on Integrating Canopy, Rootstock and Environmental Physiology in Orchard Systems. International Society for Horticultural Science. 2011. p. 87-93. (Acta Horticulturae). https://doi.org/10.17660/ActaHortic.2011.903.7