RRD1, a component of the TORC1 signalling pathway, affects anaesthetic response in Saccharomyces cerevisiae

Laura K. Palmer, Beverly A. Baptiste, John C. Fester, Justin C. Perkins, Ralph L. Keil

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The molecular mechanisms of action of volatile anaesthetics remain unknown despite clinical use for over 150 years. While many effects of these agents have been characterized, clear insight into how these effects relate to the physiological state of anaesthesia has not been established. Volatile anaesthetics arrest cell division in Saccharomyces cerevisiae in a manner that parallels the anaesthetic actions of these drugs in mammals. To gain additional insight into the cellular activities of these drugs, we isolated genes that, when present on multi-copy plasmids, render S. cerevisiae resistant to the volatile anaesthetic isoflurane. One of these genes, RRD1, encodes a subunit of the Tap42p-Sit4p-Rrd1p phosphatase complex that functions in the target of rapamycin complex 1 (TORC1) signalling pathway. In addition, we show that mutations in two other genes encoding components of the TORC1 pathway, GLN3 and URE2, also affect yeast anaesthetic response. These findings suggest that TORC1-mediated signalling is involved in cellular response to volatile anaesthetics in S. cerevisiae.

Original languageEnglish (US)
Pages (from-to)655-661
Number of pages7
JournalYeast
Volume26
Issue number12
DOIs
StatePublished - Dec 1 2009

Fingerprint

Anesthetics
Yeast
Saccharomyces cerevisiae
Genes
Gene Components
Gene encoding
Mammals
Isoflurane
Phosphatases
Phosphoric Monoester Hydrolases
Pharmaceutical Preparations
Cell Division
mechanistic target of rapamycin complex 1
Plasmids
Anesthesia
Yeasts
Cells
Mutation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

Palmer, Laura K. ; Baptiste, Beverly A. ; Fester, John C. ; Perkins, Justin C. ; Keil, Ralph L. / RRD1, a component of the TORC1 signalling pathway, affects anaesthetic response in Saccharomyces cerevisiae. In: Yeast. 2009 ; Vol. 26, No. 12. pp. 655-661.
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RRD1, a component of the TORC1 signalling pathway, affects anaesthetic response in Saccharomyces cerevisiae. / Palmer, Laura K.; Baptiste, Beverly A.; Fester, John C.; Perkins, Justin C.; Keil, Ralph L.

In: Yeast, Vol. 26, No. 12, 01.12.2009, p. 655-661.

Research output: Contribution to journalArticle

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