Abstract
Self-incompatibility (SI) in Petunia is regulated by a polymorphic S-locus. For each S-haplotype, the S-locus contains a pistilspecific S-RNase gene and multiple pollen-specific S-locus F-box (SLF) genes. Both gain-of-function and loss-of-function experiments have shown that S-RNase alone regulates pistil specificity in SI. Gain-of-function experiments on SLF genes suggest that the entire suite of encoded proteins constitute the pollen specificity determinant. However, clear-cut loss-offunction experiments must be performed to determine if SLF proteins are essential for SI of pollen. Here, we used CRISPR/ Cas9 to generate two frame-shift indel alleles of S 2 -SLF1 (SLF1 of S 2 -haplotype) in S 2 S 3 plants of P. inflata and examined the effect on the SI behavior of S 2 pollen. In the absence of a functional S 2 -SLF 1 , S 2 pollen was either rejected by or remained compatible with pistils carrying one of eight normally compatible S-haplotypes. All results are consistent with interaction relationships between the 17 SLF proteins of S 2 -haplotype and these eight S-RNases that had been determined by gain-offunction experiments performed previously or in this work. Our loss-of-function results provide definitive evidence that SLF proteins are solely responsible for SI of pollen, and they reveal their diverse and complex interaction relationships with S-RNases to maintain SI while ensuring cross-compatibility.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2959-2972 |
| Number of pages | 14 |
| Journal | Plant Cell |
| Volume | 30 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 1 2018 |
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All Science Journal Classification (ASJC) codes
- Plant Science
- Cell Biology
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S-locus F-Box proteins are solely responsible for S-RNase-based self-incompatibility of Petunia Pollen. / Sun, Linhan; Williams, Justin S.; Li, Shu; Wu, Lihua; Khatri, Wasi A.; Stone, Patrick G.; Keebaugh, Matthew D.; Kao, Teh-hui.
In: Plant Cell, Vol. 30, No. 12, 01.12.2018, p. 2959-2972.Research output: Contribution to journal › Article
TY - JOUR
T1 - S-locus F-Box proteins are solely responsible for S-RNase-based self-incompatibility of Petunia Pollen
AU - Sun, Linhan
AU - Williams, Justin S.
AU - Li, Shu
AU - Wu, Lihua
AU - Khatri, Wasi A.
AU - Stone, Patrick G.
AU - Keebaugh, Matthew D.
AU - Kao, Teh-hui
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Self-incompatibility (SI) in Petunia is regulated by a polymorphic S-locus. For each S-haplotype, the S-locus contains a pistilspecific S-RNase gene and multiple pollen-specific S-locus F-box (SLF) genes. Both gain-of-function and loss-of-function experiments have shown that S-RNase alone regulates pistil specificity in SI. Gain-of-function experiments on SLF genes suggest that the entire suite of encoded proteins constitute the pollen specificity determinant. However, clear-cut loss-offunction experiments must be performed to determine if SLF proteins are essential for SI of pollen. Here, we used CRISPR/ Cas9 to generate two frame-shift indel alleles of S 2 -SLF1 (SLF1 of S 2 -haplotype) in S 2 S 3 plants of P. inflata and examined the effect on the SI behavior of S 2 pollen. In the absence of a functional S 2 -SLF 1 , S 2 pollen was either rejected by or remained compatible with pistils carrying one of eight normally compatible S-haplotypes. All results are consistent with interaction relationships between the 17 SLF proteins of S 2 -haplotype and these eight S-RNases that had been determined by gain-offunction experiments performed previously or in this work. Our loss-of-function results provide definitive evidence that SLF proteins are solely responsible for SI of pollen, and they reveal their diverse and complex interaction relationships with S-RNases to maintain SI while ensuring cross-compatibility.
AB - Self-incompatibility (SI) in Petunia is regulated by a polymorphic S-locus. For each S-haplotype, the S-locus contains a pistilspecific S-RNase gene and multiple pollen-specific S-locus F-box (SLF) genes. Both gain-of-function and loss-of-function experiments have shown that S-RNase alone regulates pistil specificity in SI. Gain-of-function experiments on SLF genes suggest that the entire suite of encoded proteins constitute the pollen specificity determinant. However, clear-cut loss-offunction experiments must be performed to determine if SLF proteins are essential for SI of pollen. Here, we used CRISPR/ Cas9 to generate two frame-shift indel alleles of S 2 -SLF1 (SLF1 of S 2 -haplotype) in S 2 S 3 plants of P. inflata and examined the effect on the SI behavior of S 2 pollen. In the absence of a functional S 2 -SLF 1 , S 2 pollen was either rejected by or remained compatible with pistils carrying one of eight normally compatible S-haplotypes. All results are consistent with interaction relationships between the 17 SLF proteins of S 2 -haplotype and these eight S-RNases that had been determined by gain-offunction experiments performed previously or in this work. Our loss-of-function results provide definitive evidence that SLF proteins are solely responsible for SI of pollen, and they reveal their diverse and complex interaction relationships with S-RNases to maintain SI while ensuring cross-compatibility.
UR - http://www.scopus.com/inward/record.url?scp=85060591068&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85060591068&partnerID=8YFLogxK
U2 - 10.1105/tpc.18.00615
DO - 10.1105/tpc.18.00615
M3 - Article
C2 - 30377238
AN - SCOPUS:85060591068
VL - 30
SP - 2959
EP - 2972
JO - Plant Cell
JF - Plant Cell
SN - 1040-4651
IS - 12
ER -