S-locus F-Box proteins are solely responsible for S-RNase-based self-incompatibility of Petunia Pollen

Linhan Sun, Justin S. Williams, Shu Li, Lihua Wu, Wasi A. Khatri, Patrick G. Stone, Matthew D. Keebaugh, Teh-hui Kao

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Self-incompatibility (SI) in Petunia is regulated by a polymorphic S-locus. For each S-haplotype, the S-locus contains a pistilspecific S-RNase gene and multiple pollen-specific S-locus F-box (SLF) genes. Both gain-of-function and loss-of-function experiments have shown that S-RNase alone regulates pistil specificity in SI. Gain-of-function experiments on SLF genes suggest that the entire suite of encoded proteins constitute the pollen specificity determinant. However, clear-cut loss-offunction experiments must be performed to determine if SLF proteins are essential for SI of pollen. Here, we used CRISPR/ Cas9 to generate two frame-shift indel alleles of S 2 -SLF1 (SLF1 of S 2 -haplotype) in S 2 S 3 plants of P. inflata and examined the effect on the SI behavior of S 2 pollen. In the absence of a functional S 2 -SLF 1 , S 2 pollen was either rejected by or remained compatible with pistils carrying one of eight normally compatible S-haplotypes. All results are consistent with interaction relationships between the 17 SLF proteins of S 2 -haplotype and these eight S-RNases that had been determined by gain-offunction experiments performed previously or in this work. Our loss-of-function results provide definitive evidence that SLF proteins are solely responsible for SI of pollen, and they reveal their diverse and complex interaction relationships with S-RNases to maintain SI while ensuring cross-compatibility.

Original languageEnglish (US)
Pages (from-to)2959-2972
Number of pages14
JournalPlant Cell
Volume30
Issue number12
DOIs
StatePublished - Dec 1 2018

Fingerprint

F-Box Proteins
Petunia
F-box proteins
Pollen
pollen
Haplotypes
loci
haplotypes
pistil
Clustered Regularly Interspaced Short Palindromic Repeats
Genes
Protein S
genes
ribonuclease S
Alleles
alleles

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Sun, Linhan ; Williams, Justin S. ; Li, Shu ; Wu, Lihua ; Khatri, Wasi A. ; Stone, Patrick G. ; Keebaugh, Matthew D. ; Kao, Teh-hui. / S-locus F-Box proteins are solely responsible for S-RNase-based self-incompatibility of Petunia Pollen. In: Plant Cell. 2018 ; Vol. 30, No. 12. pp. 2959-2972.
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abstract = "Self-incompatibility (SI) in Petunia is regulated by a polymorphic S-locus. For each S-haplotype, the S-locus contains a pistilspecific S-RNase gene and multiple pollen-specific S-locus F-box (SLF) genes. Both gain-of-function and loss-of-function experiments have shown that S-RNase alone regulates pistil specificity in SI. Gain-of-function experiments on SLF genes suggest that the entire suite of encoded proteins constitute the pollen specificity determinant. However, clear-cut loss-offunction experiments must be performed to determine if SLF proteins are essential for SI of pollen. Here, we used CRISPR/ Cas9 to generate two frame-shift indel alleles of S 2 -SLF1 (SLF1 of S 2 -haplotype) in S 2 S 3 plants of P. inflata and examined the effect on the SI behavior of S 2 pollen. In the absence of a functional S 2 -SLF 1 , S 2 pollen was either rejected by or remained compatible with pistils carrying one of eight normally compatible S-haplotypes. All results are consistent with interaction relationships between the 17 SLF proteins of S 2 -haplotype and these eight S-RNases that had been determined by gain-offunction experiments performed previously or in this work. Our loss-of-function results provide definitive evidence that SLF proteins are solely responsible for SI of pollen, and they reveal their diverse and complex interaction relationships with S-RNases to maintain SI while ensuring cross-compatibility.",
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Sun, L, Williams, JS, Li, S, Wu, L, Khatri, WA, Stone, PG, Keebaugh, MD & Kao, T 2018, 'S-locus F-Box proteins are solely responsible for S-RNase-based self-incompatibility of Petunia Pollen', Plant Cell, vol. 30, no. 12, pp. 2959-2972. https://doi.org/10.1105/tpc.18.00615

S-locus F-Box proteins are solely responsible for S-RNase-based self-incompatibility of Petunia Pollen. / Sun, Linhan; Williams, Justin S.; Li, Shu; Wu, Lihua; Khatri, Wasi A.; Stone, Patrick G.; Keebaugh, Matthew D.; Kao, Teh-hui.

In: Plant Cell, Vol. 30, No. 12, 01.12.2018, p. 2959-2972.

Research output: Contribution to journalArticle

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T1 - S-locus F-Box proteins are solely responsible for S-RNase-based self-incompatibility of Petunia Pollen

AU - Sun, Linhan

AU - Williams, Justin S.

AU - Li, Shu

AU - Wu, Lihua

AU - Khatri, Wasi A.

AU - Stone, Patrick G.

AU - Keebaugh, Matthew D.

AU - Kao, Teh-hui

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