S9, a 19 S proteasome subunit interacting with ubiquitinated NF-κB2/p100

Abraham Fong, Minying Zhang, John Neely, Shao Cong Sun

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Proteasome-mediated processing of the nfκb2 gene product p100 is a regulated event that generates the NF-κB subunit p52. This event can be induced through p100 phosphorylation by a signaling pathway involving the nuclear factor-κB-inducing kinase (NIK). The C-terminal region of p100, which contains its phosphorylation site and a death domain, plays a pivotal role in regulating the processing of p100. To understand the biochemical mechanism of p100 processing, we searched for cellular factors interacting with the C-terminal regulatory region of p100 using the yeast two-hybrid system. This led to the identification of S9, a non-ATPase subunit of the 19 S proteasome with no known functions. Interestingly, the S9/p100 interaction could be induced by NIK but not by a catalytically inactive NIK mutant. This inducible molecular interaction required p100 ubiquitination and was dependent on the intact death domain. We further demonstrated that the death domain is essential for NIK-induced post-translational processing of p100, thus providing a functional link between the S9 binding and the processing of p100. Finally, we provide genetic evidence for the essential role of S9 in the inducible processing of p100.

Original languageEnglish (US)
Pages (from-to)40697-40702
Number of pages6
JournalJournal of Biological Chemistry
Volume277
Issue number43
DOIs
StatePublished - Oct 25 2002

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Proteasome Endopeptidase Complex
Processing
Phosphorylation
Two-Hybrid System Techniques
Nucleic Acid Regulatory Sequences
Ubiquitination
Phosphotransferases
Molecular interactions
Hybrid systems
Yeast
Genes
Death Domain

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Fong, Abraham ; Zhang, Minying ; Neely, John ; Sun, Shao Cong. / S9, a 19 S proteasome subunit interacting with ubiquitinated NF-κB2/p100. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 43. pp. 40697-40702.
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S9, a 19 S proteasome subunit interacting with ubiquitinated NF-κB2/p100. / Fong, Abraham; Zhang, Minying; Neely, John; Sun, Shao Cong.

In: Journal of Biological Chemistry, Vol. 277, No. 43, 25.10.2002, p. 40697-40702.

Research output: Contribution to journalArticle

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AU - Sun, Shao Cong

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