Sakacin a-containing pullulan film: An active packaging system to control epidemic clones of Listeria Monocytogenes in ready-to-eat foods

Valentina Trinetta, John D. Floros, Catherine Nettles Cutter

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The effectiveness of sakacin A, a bacteriocin produced by Lactobacillus sakei DSMZ 6333, was evaluated against epidemic clones of Listeria monocytogenes isolated from foodborne outbreaks; sakacin A activity was determined in ready-to-eat (RTE) foods, and a biopolymer was identified to deliver the bacteriocin onto a RTE food. Sakacin A demonstrated antimicrobial activity against epidemic clones of L. monocytogenes in food slurries. When sakacin A (50 mg/mL) was applied directly to experimentally inoculated turkey breast, populations of the most sensitive epidemic clone were reduced more than 2 log10 cfu/g after 3 weeks at 4C. Sakacin A-containing (1 mg/cm2) pullulan films demonstrated antimicrobial activity in vitro. When experimentally inoculated surfaces of turkey breast were treated with these pullulan films, L. monocytogenes populations were reduced 3 log10 cfu/g after 3 weeks under refrigerated storage. These results demonstrate the possibility of using sakacin A-containing-pullulan film to inhibit or reduce L. monocytogenes on the surface of a RTE food.

Original languageEnglish (US)
Pages (from-to)366-381
Number of pages16
JournalJournal of Food Safety
Volume30
Issue number2
DOIs
StatePublished - Jan 1 2010

Fingerprint

sakacins
pullulan
ready-to-eat foods
Listeria monocytogenes
Product Packaging
films (materials)
packaging
Clone Cells
clones
Food
Bacteriocins
bacteriocins
breasts
Breast
anti-infective agents
Lactobacillus sakei
Biopolymers
slurries
biopolymers
Vulnerable Populations

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Food Science
  • Microbiology

Cite this

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title = "Sakacin a-containing pullulan film: An active packaging system to control epidemic clones of Listeria Monocytogenes in ready-to-eat foods",
abstract = "The effectiveness of sakacin A, a bacteriocin produced by Lactobacillus sakei DSMZ 6333, was evaluated against epidemic clones of Listeria monocytogenes isolated from foodborne outbreaks; sakacin A activity was determined in ready-to-eat (RTE) foods, and a biopolymer was identified to deliver the bacteriocin onto a RTE food. Sakacin A demonstrated antimicrobial activity against epidemic clones of L. monocytogenes in food slurries. When sakacin A (50 mg/mL) was applied directly to experimentally inoculated turkey breast, populations of the most sensitive epidemic clone were reduced more than 2 log10 cfu/g after 3 weeks at 4C. Sakacin A-containing (1 mg/cm2) pullulan films demonstrated antimicrobial activity in vitro. When experimentally inoculated surfaces of turkey breast were treated with these pullulan films, L. monocytogenes populations were reduced 3 log10 cfu/g after 3 weeks under refrigerated storage. These results demonstrate the possibility of using sakacin A-containing-pullulan film to inhibit or reduce L. monocytogenes on the surface of a RTE food.",
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Sakacin a-containing pullulan film : An active packaging system to control epidemic clones of Listeria Monocytogenes in ready-to-eat foods. / Trinetta, Valentina; Floros, John D.; Cutter, Catherine Nettles.

In: Journal of Food Safety, Vol. 30, No. 2, 01.01.2010, p. 366-381.

Research output: Contribution to journalArticle

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