Salivary glucose oxidase: Multifunctional roles for Helicoverpa zea?

Herbert Eichenseer, M. Claravon Mathews, Jian L. Bi, J. Brad Murphy, Gary Felton

Research output: Contribution to journalArticlepeer-review

137 Scopus citations

Abstract

Labial glands of Helicoverpa zea produced a glucose oxidase (GOX), which was present in the saliva and midgut lumen. We purified GOX 23-fold by isoelectric focusing of labial gland homogenates and investigated physical and kinetic properties of the enzyme. D-glucose and 6-deoxy-D-glucose were the optimal substrates of 22 carbohydrates tested with GOX. The enzyme was not inhibited by several inhibitors of fungal GOX but was sensitive to HgCl2. Labial gland GOX activities varied daily during larval development with highest activities found when larvae were actively feeding. Based on pH optimum, pI, molecular weight estimate and Km(glucose), the insect enzyme is not derived from fungal GOXs but appears to have similar kinetic and physical attributes to other insect GOXs. Some possible functions are discussed, including antimicrobial properties, manipulating midgut O2 levels, altering host plant defense responses, and metabolizing carbohydrates.

Original languageEnglish (US)
Pages (from-to)99-109
Number of pages11
JournalArchives of Insect Biochemistry and Physiology
Volume42
Issue number1
DOIs
StatePublished - Jan 1 1999

All Science Journal Classification (ASJC) codes

  • Physiology
  • Biochemistry
  • Insect Science

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