Salmonella newport contamination of produce

Ecological, genetic and epidemiological aspects

Rebecca L. Bell, Guojie Cao, Jianghong Meng, Marc W. Allard, Christine Keys, Thomas Hill, Andrea Ottesen, Eric W. Brown

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Salmonella enterica is the most frequent source of bacterial foodborne illness in the U.S., causing an estimated 1.4 million cases of salmonellosis annually. Historically, infection by Salmonella occurs after eating undercooked meats, poultry and eggs. However, over the past decade, the consumption of raw ready-to-eat produce has been implicated in several severe and widespread outbreaks within the U. S. Most notably, an S. enterica Saintpaul outbreak associated with jalapeňo and Serrano peppers sickened over 1400 individuals during the summer of 2008. One Salmonella serovar in particular, Salmonella enterica Newport, has been associated perennially with contamination events involving fresh tomatoes and prepared fresh foods containing tomatoes. S. Newport is a non-typhoidal salmonellae classified taxonomically as subspecies (i.e. "group") I Salmonella enterica enterica. Prior to its emergence in produce and in produce growing environments, S. Newport was found predominantly in swine, cattle and other livestock commodities. S. Newport association with tomatoes retains a geographically skewed distribution. Metagenomic analyses of tomato phyllopsheres, based on 16S rDNA sequences and deep genome sequencing of tomato microbial communities, point to resident colonization on tomatoes from the Mid-Atlantic growing regions but not other predominant fresh tomato growing regions such as Florida or California, signaling niche adaptation of S. Newport in some growing environments but not others. Genetic analysis of S. Newport has revealed specific differences among strains from disparate locales. Pulsed-field gel electrophoresis, for example, has yielded several pulsotypes of environmental S. Newport primarily associated with the Virginia eastern shore, a prominent fresh tomato production region of the county. Whole-genome sequencing affirmed these phylo-geographic differences within the serovar. Surprisingly, certain genotypes of S. Newport have been isolated from this region that genetically match previous outbreaks where tomatoes were a suspected vehicle. S. Newport can be readily detected in the environment of the Virginian eastern shore growing region using both molecular (i.e. PCR) and microbiological (i.e. FDA-BAM) screening methods. Taken together, these issues highlight the important intersection of microbiological, genetic, and ecological investigation for fully understanding the complex life cycle of this dangerous foodborne pathogen.

Original languageEnglish (US)
Title of host publicationSalmonella: Classification, Genetics and Disease Outbreaks
PublisherNova Science Publishers, Inc.
Pages155-174
Number of pages20
ISBN (Print)9781619429284
StatePublished - 2012

Fingerprint

Lycopersicon esculentum
Salmonella
Salmonella enterica
Disease Outbreaks
Salmonella Infections
Mid-Atlantic Region
Genome
High-Throughput Nucleotide Sequencing
Metagenomics
Foodborne Diseases
Pulsed Field Gel Electrophoresis
Livestock
Poultry
Ribosomal DNA
Life Cycle Stages
Meat
Eggs
Swine
Eating
Genotype

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Bell, R. L., Cao, G., Meng, J., Allard, M. W., Keys, C., Hill, T., ... Brown, E. W. (2012). Salmonella newport contamination of produce: Ecological, genetic and epidemiological aspects. In Salmonella: Classification, Genetics and Disease Outbreaks (pp. 155-174). Nova Science Publishers, Inc..
Bell, Rebecca L. ; Cao, Guojie ; Meng, Jianghong ; Allard, Marc W. ; Keys, Christine ; Hill, Thomas ; Ottesen, Andrea ; Brown, Eric W. / Salmonella newport contamination of produce : Ecological, genetic and epidemiological aspects. Salmonella: Classification, Genetics and Disease Outbreaks. Nova Science Publishers, Inc., 2012. pp. 155-174
@inbook{aab40cf1d5724cca95944d531c282ddb,
title = "Salmonella newport contamination of produce: Ecological, genetic and epidemiological aspects",
abstract = "Salmonella enterica is the most frequent source of bacterial foodborne illness in the U.S., causing an estimated 1.4 million cases of salmonellosis annually. Historically, infection by Salmonella occurs after eating undercooked meats, poultry and eggs. However, over the past decade, the consumption of raw ready-to-eat produce has been implicated in several severe and widespread outbreaks within the U. S. Most notably, an S. enterica Saintpaul outbreak associated with jalapeňo and Serrano peppers sickened over 1400 individuals during the summer of 2008. One Salmonella serovar in particular, Salmonella enterica Newport, has been associated perennially with contamination events involving fresh tomatoes and prepared fresh foods containing tomatoes. S. Newport is a non-typhoidal salmonellae classified taxonomically as subspecies (i.e. {"}group{"}) I Salmonella enterica enterica. Prior to its emergence in produce and in produce growing environments, S. Newport was found predominantly in swine, cattle and other livestock commodities. S. Newport association with tomatoes retains a geographically skewed distribution. Metagenomic analyses of tomato phyllopsheres, based on 16S rDNA sequences and deep genome sequencing of tomato microbial communities, point to resident colonization on tomatoes from the Mid-Atlantic growing regions but not other predominant fresh tomato growing regions such as Florida or California, signaling niche adaptation of S. Newport in some growing environments but not others. Genetic analysis of S. Newport has revealed specific differences among strains from disparate locales. Pulsed-field gel electrophoresis, for example, has yielded several pulsotypes of environmental S. Newport primarily associated with the Virginia eastern shore, a prominent fresh tomato production region of the county. Whole-genome sequencing affirmed these phylo-geographic differences within the serovar. Surprisingly, certain genotypes of S. Newport have been isolated from this region that genetically match previous outbreaks where tomatoes were a suspected vehicle. S. Newport can be readily detected in the environment of the Virginian eastern shore growing region using both molecular (i.e. PCR) and microbiological (i.e. FDA-BAM) screening methods. Taken together, these issues highlight the important intersection of microbiological, genetic, and ecological investigation for fully understanding the complex life cycle of this dangerous foodborne pathogen.",
author = "Bell, {Rebecca L.} and Guojie Cao and Jianghong Meng and Allard, {Marc W.} and Christine Keys and Thomas Hill and Andrea Ottesen and Brown, {Eric W.}",
year = "2012",
language = "English (US)",
isbn = "9781619429284",
pages = "155--174",
booktitle = "Salmonella: Classification, Genetics and Disease Outbreaks",
publisher = "Nova Science Publishers, Inc.",

}

Bell, RL, Cao, G, Meng, J, Allard, MW, Keys, C, Hill, T, Ottesen, A & Brown, EW 2012, Salmonella newport contamination of produce: Ecological, genetic and epidemiological aspects. in Salmonella: Classification, Genetics and Disease Outbreaks. Nova Science Publishers, Inc., pp. 155-174.

Salmonella newport contamination of produce : Ecological, genetic and epidemiological aspects. / Bell, Rebecca L.; Cao, Guojie; Meng, Jianghong; Allard, Marc W.; Keys, Christine; Hill, Thomas; Ottesen, Andrea; Brown, Eric W.

Salmonella: Classification, Genetics and Disease Outbreaks. Nova Science Publishers, Inc., 2012. p. 155-174.

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Salmonella newport contamination of produce

T2 - Ecological, genetic and epidemiological aspects

AU - Bell, Rebecca L.

AU - Cao, Guojie

AU - Meng, Jianghong

AU - Allard, Marc W.

AU - Keys, Christine

AU - Hill, Thomas

AU - Ottesen, Andrea

AU - Brown, Eric W.

PY - 2012

Y1 - 2012

N2 - Salmonella enterica is the most frequent source of bacterial foodborne illness in the U.S., causing an estimated 1.4 million cases of salmonellosis annually. Historically, infection by Salmonella occurs after eating undercooked meats, poultry and eggs. However, over the past decade, the consumption of raw ready-to-eat produce has been implicated in several severe and widespread outbreaks within the U. S. Most notably, an S. enterica Saintpaul outbreak associated with jalapeňo and Serrano peppers sickened over 1400 individuals during the summer of 2008. One Salmonella serovar in particular, Salmonella enterica Newport, has been associated perennially with contamination events involving fresh tomatoes and prepared fresh foods containing tomatoes. S. Newport is a non-typhoidal salmonellae classified taxonomically as subspecies (i.e. "group") I Salmonella enterica enterica. Prior to its emergence in produce and in produce growing environments, S. Newport was found predominantly in swine, cattle and other livestock commodities. S. Newport association with tomatoes retains a geographically skewed distribution. Metagenomic analyses of tomato phyllopsheres, based on 16S rDNA sequences and deep genome sequencing of tomato microbial communities, point to resident colonization on tomatoes from the Mid-Atlantic growing regions but not other predominant fresh tomato growing regions such as Florida or California, signaling niche adaptation of S. Newport in some growing environments but not others. Genetic analysis of S. Newport has revealed specific differences among strains from disparate locales. Pulsed-field gel electrophoresis, for example, has yielded several pulsotypes of environmental S. Newport primarily associated with the Virginia eastern shore, a prominent fresh tomato production region of the county. Whole-genome sequencing affirmed these phylo-geographic differences within the serovar. Surprisingly, certain genotypes of S. Newport have been isolated from this region that genetically match previous outbreaks where tomatoes were a suspected vehicle. S. Newport can be readily detected in the environment of the Virginian eastern shore growing region using both molecular (i.e. PCR) and microbiological (i.e. FDA-BAM) screening methods. Taken together, these issues highlight the important intersection of microbiological, genetic, and ecological investigation for fully understanding the complex life cycle of this dangerous foodborne pathogen.

AB - Salmonella enterica is the most frequent source of bacterial foodborne illness in the U.S., causing an estimated 1.4 million cases of salmonellosis annually. Historically, infection by Salmonella occurs after eating undercooked meats, poultry and eggs. However, over the past decade, the consumption of raw ready-to-eat produce has been implicated in several severe and widespread outbreaks within the U. S. Most notably, an S. enterica Saintpaul outbreak associated with jalapeňo and Serrano peppers sickened over 1400 individuals during the summer of 2008. One Salmonella serovar in particular, Salmonella enterica Newport, has been associated perennially with contamination events involving fresh tomatoes and prepared fresh foods containing tomatoes. S. Newport is a non-typhoidal salmonellae classified taxonomically as subspecies (i.e. "group") I Salmonella enterica enterica. Prior to its emergence in produce and in produce growing environments, S. Newport was found predominantly in swine, cattle and other livestock commodities. S. Newport association with tomatoes retains a geographically skewed distribution. Metagenomic analyses of tomato phyllopsheres, based on 16S rDNA sequences and deep genome sequencing of tomato microbial communities, point to resident colonization on tomatoes from the Mid-Atlantic growing regions but not other predominant fresh tomato growing regions such as Florida or California, signaling niche adaptation of S. Newport in some growing environments but not others. Genetic analysis of S. Newport has revealed specific differences among strains from disparate locales. Pulsed-field gel electrophoresis, for example, has yielded several pulsotypes of environmental S. Newport primarily associated with the Virginia eastern shore, a prominent fresh tomato production region of the county. Whole-genome sequencing affirmed these phylo-geographic differences within the serovar. Surprisingly, certain genotypes of S. Newport have been isolated from this region that genetically match previous outbreaks where tomatoes were a suspected vehicle. S. Newport can be readily detected in the environment of the Virginian eastern shore growing region using both molecular (i.e. PCR) and microbiological (i.e. FDA-BAM) screening methods. Taken together, these issues highlight the important intersection of microbiological, genetic, and ecological investigation for fully understanding the complex life cycle of this dangerous foodborne pathogen.

UR - http://www.scopus.com/inward/record.url?scp=84892025585&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84892025585&partnerID=8YFLogxK

M3 - Chapter

SN - 9781619429284

SP - 155

EP - 174

BT - Salmonella: Classification, Genetics and Disease Outbreaks

PB - Nova Science Publishers, Inc.

ER -

Bell RL, Cao G, Meng J, Allard MW, Keys C, Hill T et al. Salmonella newport contamination of produce: Ecological, genetic and epidemiological aspects. In Salmonella: Classification, Genetics and Disease Outbreaks. Nova Science Publishers, Inc. 2012. p. 155-174