Salmonella promotes virulence by repressing cellulose production

Mauricio Henriques Pontes, Eun Jin Lee, Jeongjoon Choi, Eduardo A. Groisman

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Cellulose is the most abundant organic polymer on Earth. In bacteria, cellulose confers protection against environmental insults and is a constituent of biofilms typically formed on abiotic surfaces. We report that, surprisingly, Salmonella enterica serovar Typhimurium makes cellulose when inside macrophages. We determine that preventing cellulose synthesis increases virulence, whereas stimulation of cellulose synthesis inside macrophages decreases virulence. An attenuated mutant lacking the mgtC gene exhibited increased cellulose levels due to increased expression of the cellulose synthase gene bcsA and of cyclic diguanylate, the allosteric activator of the BcsA protein. Inactivation of bcsA restored wild-type virulence to the Salmonella mgtC mutant, but not to other attenuated mutants displaying a wild-type phenotype regarding cellulose. Our findings indicate that a virulence determinant can promote pathogenicity by repressing a pathogen's antivirulence trait. Moreover, they suggest that controlling antivirulence traits increases long-term pathogen fitness by mediating a trade-off between acute virulence and transmission.

Original languageEnglish (US)
Pages (from-to)5183-5188
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number16
DOIs
StatePublished - Apr 21 2015

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Cellulose
Salmonella
Virulence
Macrophages
Salmonella enterica
Conservation of Natural Resources
Biofilms
Genes
Polymers
Bacteria
Phenotype
Proteins

All Science Journal Classification (ASJC) codes

  • General

Cite this

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Salmonella promotes virulence by repressing cellulose production. / Pontes, Mauricio Henriques; Lee, Eun Jin; Choi, Jeongjoon; Groisman, Eduardo A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, No. 16, 21.04.2015, p. 5183-5188.

Research output: Contribution to journalArticle

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AU - Pontes, Mauricio Henriques

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